Studies On Ingredients From Two Marine-derived Fungi

Objective:in order to fully explore the chemical structure and biological activity diversity of secondary metabolites of marine mangrove derived fungi and give full play to the research value and application prospect of marine derived natural products,2 marine mangrove derived fungi Trichoderma asperellum H553 and Penicillium granulatum H26 were considered as the target strain and the chemical composition is studied in this paper.Methods:the chemical composition of 2 fungi were analyzed by the methods of silica gel column chromatography,reversed-phase ODS column chromatography,thinlayer chromatography and high performance liquid chromatography combined with 1D/2D-NMR and MS.Results:for T.asperellum H553,its crude extract can inhibit the expression level of MUC1 in MDA-MB-468-MUC1 and BEL-7402-MUC1 stable transfected tumor cell line,which is a potential target fungi for MUC1 protein,then 20 compounds have been isolated and purified,5 compounds are phthalates,1 compound is indene derivative,3 compounds are alkaloids,2 compounds are cyclic peptides,1 compound is pyran derivative,6 compounds are terpenes and 2 compounds are sterols,of which 3 compound are novel compounds.For P.granulatum H26,14 compounds were isolated and purified,1 compound is biphenyl,1 compound is polyketone,3 compounds are sterols,1 compound is phthalide,1 compound is phthalate,4 compounds are benzopyrones,2 are pyran derivatives,1 compound is furanone which is a novel compound.Some of the compounds from T.asperellum H553 and P.granulatum H26 were screened for their antitumor activity.Compound 1,5,6 and 8 has good cytotoxic activity against MDA-MB-468 and Bel-7402.Conclusion:32 compounds were isolated from T.asperellum h553 and P.granulatum H26,of which 4 compounds are novel compounds and their biological activities were evaluated.