Fermentation Culture And Metabolite Constituents Of The Penicillium Verruculosum Yl-52 Isolated From The Rhizosphere Of Stellera Chamaejasme L.

The root of Stellera chamaejasme L. is poisonous, so it is a special environment for microorganisms. There are many microorganisms which may produce metabolites with particular chemical structure and biological activity in it's rhizosphere. Our team has found a strain with high antimicrobial activity, and identified it as Penicillium verruculosum YL-52. This paper is based on preliminary studies, and studies the metabolite contituents.of the strain by liquid fermentation.80 liter fermentation broth was gotten by activation, making fungal suspension and liquid fermentation for YL-52. After air pump filtration, extraction and concentration, it was devided into four parts: petroleum ether extract 12.5 g, ethyl acetate extract 18.0 g, butanol extract 25.2 g, water part 30.4 g.The TLC systematic pretest of ethyl acetate extract showed that there are volatile oils, flavonoids, terpins, steroid saponins and its glycosides, phenils, cumarins and lactones, organic acids.Five compounds were isolated and purified from ethyl acetate extract by D101 macroporous adsorption resins, silica gel, Sephedex LH-20 column chromatography and the silica gel column and layer preparation. Based on the analysis of their spectral data (IR, MS, 1H NMR, 13C NMR) and compared with the related literatural data, two compounds were identified as evernic acid(CH-2), ursolic acid(CH-3). They were both firstly found from the Penicillium verruculosum.