The Study Of Lymphocyte Subsets And Cytokines In Chronic Dacryocystitis And The Exploration Of Reparation Of Lacrimal Duct Mucosa

Section 1 The imbalance of lymphocyte subsets and cytokines in chronic dacryocystitisPurpose:To explore the quantitative distributions of different lymphocyte subsets in the lacrimal sac mucosa and identify changes of Th1-and Th2-associated cytokines in the tears of patients with chronic dacryocystitis.Methods:Lacrimal sac mucosal specimens from patients with chronic dacryocystitis were analyzed.Hematoxylin-eosin(H&E)staining and Masson staining were performed for pathological analysis,and immunohistochemical staining was performed for the detection of CD3~+,CD4~+,CD8~+,CD20~+,Th1 and Th2 lymphocytes.Quantitative real-time PCR was performed to analyze IFN-γand IL-4 m RNA expression.In addition,tear samples from patients with chronic dacryocystitis and healthy volunteers were collected and analyzed with an antibody array system for Th1-and Th2-associated cytokines and chemokines.Results:Different distribution patterns of lymphocyte subsets were observed in the lacrimal sac walls.Both CD20~+B lymphocytes and CD3~+T lymphocytes accumulated in organized lymphoid follicles,and CD3~+T cells were also distributed in a diffuse manner.Among the two subsets of T cells,CD4~+T cells were more abundant than CD8~+T cells.Both the immunohistochemical staining and real-time PCR results revealed significantly higher expression levels of IFN-γthan those of IL-4.The levels of Th1-and Th2-related cytokines and chemokines measured were significantly higher in the tears of patients than in those of controls.Conclusions:The different distribution patterns of lymphocyte subsets provide insight into a potential immunological mechanism for dacryocystitis.The cytokines secreted by Th1 or Th2 cells may play a major role in the pathogenesis of dacryocystitis and could be explored as therapeutic targets.Section 2 The exploration of reparation of lacrimal duct mucosaPurpose: To investigate the methods of establishment of lacrimal duct mucosa injury model and the effect of basic fibroblast growth factor on the proliferation of lacrimal epithelial cells and fibroblasts.Methods: Conduct lacrimal duct mucosa injury models of rabbits by laser and acid burns and perform histopathological examination.Culture epithelial cells and fibroblasts of lacrimal duct and detect the proliferation effects of b FGF on these cells by MTT assay.Results: The lacrimal duct mucosa injury model can be induced by both laser and acid burns.BFGF can promote the proliferation of lacrimal epithelial cells and fibroblasts.Conclusions: The establishment of lacrimal duct mucosa injury model will be helpful for the study of mucosal repair of lacrimal duct.BFGF has the role of promoting the proliferation of lacrimal passage cells and may have the role of promoting mucosal repairation.