The Intervention Effect Of The Extract Of Solanum Lyratum On The Epithelial Mesenchymal Transition Process Of Human Lung Adenocarcinoma Cell A549 Induced By TGF-β₁

Objective To explore whether the extract of Solanum lyratum Thunb(SLT)has impact on Epithelial Mesenchymal Transition(EMT)process of human lung adenoca rcinoma cells A549,which induced in vitro by Transforming Growth Gactor beta 1(TGF-β1).Methods Using alcohol or water to extract spreading solanum lyratum,then deal with different concentrations of solanum lyratum,CCK8 method to detect each cell inhibition rate,drawing cell growth curves,and the inhibition rate of cell growth curve.Use 5ng/ml TGF-β1 induce A549 cells for 24h,during the abduction of TGF-β1,observing the cell morphology under the inverted microscope;And use flow cytometry technology to detect the influence of concentration of SLT on cell apoptosis.Further quantitative study on effect of the extract on A549 cells the protein of epithelial marker(E-cadherin)and mesenchymal cell marker(Vimentin)by Western-Blot was detected by Western-Blot.Results 1.5ng/ml TGF-β1 lead A549 cell to morphological changes,and make the down-expression of Vimentin protein.2.CCK8 results showed that SLT provided material and alcohol extract can have a significant effect on A549 cell growth,and compared with the effect of the 24h the 48h can obviously induced to apoptosis;3.Flow cytometry detection instrument that effects of extracts of SLT on A549 cell EMT mainly lies in the inhibition of cell and cell apoptosis also have a role in promoting;4.Compared with TGF-β1 model group,after intervene by different concentration of SLT,the expression of E-cadherin is rise when Vimentin down.Conclusion1 The cell proliferation inhibition of SLT on A549 cells is closely related to concentration and time dependence.2.5ng/ml TGF-β1 A549 cells for 24 hours,can be successfully established EMT model cell.3.The extrat of SLT could reduce the expression of Vimentin and raise the expression of E-cadherin during the A549 cells epithelial meschymal transition,interference it bySLT can reduce its Show SLT can affect the process of EMT by some unknow pathway and play a role of anti-tumor.