The Relationship Between The Expression Level Of Long Non-coding RNAs And The Occurrence And Development Of Crohn’s Disease

Background:Crohn’s disease（CD）is a chronic inflammatory disease of the digestive tract.The underlying molecular mechanism of CD remains unclear.The aim of this study was to investigate the differentially expressed long non-coding RNA（lnc RNA）in CD and its possible mechanism,and to verify the expression of lnc RNA.Methods:Microarray GSE67106 and GSE83448 were downloaded from the Gene Expression Omnibus（GEO）database.Differentially expressed lnc RNAs（DELs）and messenger RNAs（m RNAs,DEGs）,when normalized through the betaqn package in the R,were determined via the limma package.Gene Ontology（GO）and Kyoto Encyclopedia of genes and genomes（KEGG）pathways were studied using the database for the annotation,visualization and integrated discovery（DAVID）version6.7,along with Gene Set Enrichment Analysis（GSEA）version 3.0.The co-expression of lnc RNAs-m RNAs were determined using weighted gene co-expression network analysis（WGCNA）.The micro RNAs（mi RNAs）related to the DELs and DEGs were forecast.A competing endogenous RNA（ce RNA）network was established.Results:There were 42 DELs and 551 DEGs identified in total among the samples of the CD and normal control,respectively.These DEGs were enriched in such pathways as retinol metabolism,renin angiotensin system,and maturation-related signaling pathways.A lnc RNA-m RNA co-expression network was constructed by WGCNA,with CDKN2B-AS（ANRIL）,CTC-210G5.1.1,RP11-467L20.10.1,RP11-325F22.5.1,and RP11-59E19.1.1 as hub DELs.Together with mi RNAs,a ce RNA network was constructed and functional analysis showed that the cell brush border and plasma membrane,synthesis and transport of lipoprotein,and angiotensin maturation,metabolism,and regulation of blood pressure were involved in the progression of CD.We successfully validated 1 lnc RNA ANRIL,in our clinical specimens,ANRIL,which can feature prominently in CD.However,the exact mechanism of lnc RNA ANRIL in CD prediction and diagnosis requires further exploration.Conclusions:This study showed that lnc RNA ANRIL has a certain predictive effect on CD occurrence and development and could be a new potential treatment target.