| Objectives To measure the serum adiponectin(APN)level before and after oral high-fat meal load in metabolic syndrome(MS)and healthy controls.To investigate the effect of acute elevation of serum triglyceride(TG)on APN secretion.Methods From May 2019 to December 2019,we recruited volunteers aged from 25 to 65 years old with confirmed metabolic syndrome from the outpatient department of Endocrinology,Hebei General Hospital.On the grounds of the exclusion criteria and inclusion criteria,a series of 49 volunteers were included in the study.At the same time,we selected 84 healthy subjects with matched gender and age in our medical checkup center as the healthy control.All the volunteers signed the informed consent.Routine physical examination and oral glucose tolerance test(OGTT)were performed.All volunteers underwent normal diet washout for one week before the high-fat meal test and fasting overnight for more than 8 hours,and eating the high-fat meal(the total calories are1500 kcal,of which,protein account for 20%,carbohydrate account for 20% and fat accounts for 60%)prepared by professional nutritionists in our hospital in the morning of the next day within 10 minutes.Venous blood samples of 0,2,4,6h after a high-fat meal were taken.Serum was separated and stored in-80℃ refrigerator for the detection of biochemical indicators and APN level.Serum APN was measured with the enzyme-linked immunosorbent(ELISA)method.SPSS25.0 software was used for statistical analysis.Results 1 There was no significant difference in gender and age between the control group and the MS group(P>0.05).Body mass index(BMI),waist circumference,diastolic blood pressure and systolic blood pressure in MS group were higher than that in the control group,and the differences were statistically significant(P<0.01).2 Fasting plasma glucose(FPG),2 hours postprandial blood glucose,fasting insulin(FINS),and homeostasis model assessment of insulin resistance(HOMA-IR)in the MS group were significantly higher than that in control group(P<0.01).3 There was no statistical difference in fasting total cholesterol(TC)between MS group and control group(P>0.05).Compared with control group,the level of fasting TG,low density lipoprotein cholesterin(LDL-C),and apolipoprotein B(Apo B)in MS group were significantly higher(P<0.01).Fasting high density lipoprotein cholesterol(HDL-C)and apolipoprotein A1(Apo A1)in MS group were all lower than in control group,and the differences were statistically significant(P<0.01).4 The fasting serum APN level in MS group was significantly lower than in control group(P<0.01).5 The TG level of fasting and 2,4,6h after high-fat meal in MS group were higher than in control group,and the differences between groups at each time point were statistically significant(P<0.01).In control group,TG peaked at 2h after highfat meal and then began to decline,but was still significantly higher than fasting TG level at 6h after the meal(P<0.01).In MS group,TG level gradually increased within 6h after high-fat meal,and TG level at all time points after high-fat meal were significantly higher than fasting TG level(P<0.01).The area increment under TG curve after fat meal in MS group was higher than that in control group,and the difference was statistically significant(P<0.01).6 Compared with control group,the level of serum APN in MS group were significantly lower at fasting and 2,4,6h after high-fat meal(P<0.01).The APN level in both groups increased first and then decreased after eating the high-fat meal,and the peak was 4h after high-fat meal,but it was still significantly higher than fasting APN level at 6h after high-fat meal(P<0.01).7 Correlation analysis: fasting serum APN level was negatively correlated with waist circumference,systolic blood pressure,diastolic blood pressure,fasting blood glucose,2h postprandial blood glucose,and fasting TG level(r=-0.492,P<0.001;r=-0.447,P<0.001;r=-0.417,P<0.001;r=-0.364,P<0.001;r=-0.290,P= 0.001;r=-0.936,P<0.001),and was positively correlated with fasting HDL-C level(r=0.415,P<0.001).In addition,serum APN level was also negatively correlated with metabolism-related indicators including BMI,insulin,HOMA-IR,fasting LDL-C,fasting TC,and fasting Apo B level(r=-0.463,P<0.001;r=-0.341,P=0.001;r=-0.402,P<0.001;r=-0.359,P<0.001;r=-0.314,P<0.001;r=-0.512,P<0.001),and positively correlated with fasting Apo A1 level(r=0.205,P= 0.018).8 Multivariate linear regression analysis was used to analyze the influencing factors of fasting serum APN,showing that fasting TG and increment in area under TG curve after meal were important influencing factors of fasting serum APN(P<0.05).Conclusions 1 Serum APN level was lower in MS patients than in healthy controls;fasting serum APN was negatively correlated with waist circumference,systolic blood pressure,diastolic blood pressure,fasting TG,fasting blood glucose,2h postprandial blood glucose,and positively correlated with fasting HDL-C level.2.The fasting TG level and the area increment under the postprandial TG curve were the important factors affecting the fasting APN level.Figure 2;Table 7;Reference 183... |
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