Study On The Relationship Between SLC30A8 Gene Polymorphism And Type 2 Diabetes In Mongolian Population

Objective: SLC30A8 gene can regulate liver insulin clearance and participate in the pathogenesis of type 2 diabetes.This study intends to screen the related risk factors and protective factors of Mongolian people with type 2 diabetes.SLC30A8 genotyping is to analyze the association between SLC30A8 gene locus and the genetic susceptibility of Mongolian type 2 diabetes.To explore the impact of the interaction between the SLC30A8 gene and the environment on the Mongolian type 2 diabetes population.It is planned to explore the pathogenesis of type 2 diabetes at the genetic level and provide a scientific basis for the development of Mongolian type 2 diabetes intervention policy in Inner Mongolia.Methods: The case group was derived from Mongolian type 2 diabetes patients in a cross-sectional study of the National Natural Science Foundation of China.The control group came from healthy people who were matched proportionally by age and gender during the same period.The case group and the control group each had 238 cases.Epidata 3.1 software enters the data,uses improved Multi-Ligase Detection Reaction to detect SLC30A8 gene SNP locus polymorphism,Plink 1.07 software analyzes Hardy-Weinberg genetic balance,SHE sis online platform software analyzes SNP locus linkage disequilibrium and Haplotype,and the generalized multi-factor dimensionality reduction method was used to analyze the interaction between the SLC30A8 gene of Mongolian type 2 diabetes and the environment.Results:(1)A total of 476 subjects were included in this study,including 240 males(50.42%)and 236 females(49.58%).The age of the case group was(56.74±10.49)years old,and the age of the control group was(57.05±10.29)years old.There was no significant difference in age and gender between the case group and the control group(P>0.05).Logistic regression analysis showed that compared with college level and above,people with junior high school,high school,elementary school level and below had a lower risk of T2DM(OR<1,P<0.05),stroke disease history,family history of diabetes,Overweight/obesity,and elevated triglyceride levels are all risk factors for Mongolian type 2 diabetes patients(OR>1,P<0.05).(2)The allele frequencies at rs13266634,rs2466293,and rs1995222 of SLC30A8 gene comply with the Hardy-Weinberg genetic balance law,with P values of 0.471,0.370,and0.500 respectively.After adjusting for confounding factors such as BMI and WHR,individuals carrying the T allele at rs13266634 have a lower risk of T2 DM compared with individuals carrying the C allele(OR=0.551,95% CI=0.350,0.868,P=0.010).Both the co-dominant model and the recessive model were found to be associated with Mongolian type2 diabetes(P<0.05).The risk of T2 DM in individuals with GA genotype at rs2466293 was2.003 times that of individuals with AA genotype(P=0.049).The rs1995222 locus has no association with type 2 diabetes in the dominant,recessive,and codominant genetic models.(3)The three SNP loci have different degrees of linkage disequilibrium.The haplotype analysis results show that haplotype CAT may increase the risk of T2DM(OR=1.694,95%CI=1.076,2.667,P= 0.022),and haplotype TAT may reduce the risk of type 2 diabetes(OR=0.659,95% CI=0.501,0.867,P=0.003).(4)The analysis of SLC30A8 gene-environment interaction by the generalized multi-factor dimensionality reduction method found that the interaction model between rs1995222 and age,occupation,education level,and physical activity was statistically significant(P=0.0107).Conclusion: Education level,history of stroke,family history of diabetes,BMI and elevated triglyceride levels may be risk factors for type 2 diabetes in Mongolian population.The rs13266634 polymorphism of SLC30A8 gene is associated with susceptibility to T2 DM.CAT and TAT are susceptible haplotypes of type 2 diabetes.The interaction of rs1995222 with age,occupation,education level,and physical activity may increase the risk of type 2 diabetes.