| Objective:To compare the differences in intestinal flora and clinical indicators in patients with polycystic ovary syndrome before and after treatment,and in healthy controls,and to investigate the potential mechanisms of intestinal flora in the development of it.Methods:Thirty newly diagnosed patients with polycystic ovary syndrome who were admitted to the Gynecological Endocrinology Clinic of Shanxi Maternal and Child Health Hospital from August 2019 to October 2020 were selected as the pre-treatment group of PCOS in this study.After 3 months of treatment,they were treated as the post-treatment group.Thirty women who underwent pre-pregnancy examination or routine physical examination in our hospital during the same period were selected as the healthy control group.Clinical data,blood and fecal samples were collected in each group.Liver function,blood glucose,blood lipids and sex hormones were determined by chemiluminescence immunoassay.The serum inflammatory indexes were as follows: Tumor necrosis factor-alpha(TNF-α),Lipopolysaccharide(LPS),interleukin-6(IL-6)were detected by ELISA.The characteristics of intestinal flora in fecal samples were analyzed by 16 SrRNA sequencing technology.Results:1.There were no significant differences in age,height,hip circumference and FSH among the three groups(P > 0.05).Compared with the control group,body weight,BMI,AST,ALT,CHO and TG were increased before treatment,and there was no statistically significant difference(P > 0.05),while waist circumference,WHR,LDLD,LH,LH/FSH,T,FPG and FINS were significantly increased(P < 0.05).2.Compared with the control group,body weight and WHR decreased slightly after treatment,while AST,ALT,CHO,TG,LH/FSH,T,FPG,FINS and LDLD increased slightly,with no statistical significance(P > 0.05).3.Compared with before treatment,body weight,WHR,LH,LH/FSH,T,FPG,FINS and LDLD decreased significantly after treatment,with statistical significance(P <0.05).4.Serum inflammatory indexes of the subjects in the three groups were detected,and compared with the control group,the three indexes before treatment were all increased,with statistical significance(P < 0.05).Compared with the control group,all three indexes decreased after treatment,but only the difference level of LPS was statistically significant(P < 0.05).Compared with before treatment,all three indexes decreased after treatment,but only TNF-α and IL-6 levels showed statistical significance(P < 0.05).5.Compared with the control group,Alpha and Beta diversity indices were decreased before and after treatment.Compared with before treatment,the above indexes increased slightly after treatment.However,there was no statistical significance in the above indicators among the three groups(P > 0.05).6.The level composition and distribution of intestinal microflora among the three groups: the dominant bacteria were Firmicutes,Bacteroidetes,Proteobacteria and Actinobacteria.The proportion in the pre-treatment group was about 67%,27%,1.5% and0.7%,respectively.In the group after treatment,the proportion was about 80%,16%,10%,0.7%;In the control group,the proportion was about: 72%,15%,7%,3%.7.At the genus level,the top five bacteria in the three groups were Faecalibacterium,Bacteroides,Coprococcus,Gemmiger and Blaotia.In the pre-treatment group,the proportion was about 30%,17%,9%,3%,3%;In the group after treatment,the proportion was about 12%,11%,11%,6%,7%;In the control group,the proportion was about: 13%,14%,13%,7%,6%.8.Bacteroidetes were positively correlated with BMI,T,LH/FSH,LPS,TNF-α and IL-6(P < 0.05),while Firmicutes were negatively correlated with BMI,FINS,FPG,LPS,TNF-α and IL-6(P < 0.05).Conclusion:Bacteroidetes were positively correlated with LPS,TNF-α and IL-6,while Firmicutes were negatively correlated with LPS.It is suggested that the increase of Bacteroidetes and the decrease of Firmicutes may destroy the intestinal mucosal barrier and cause chronic low-grade inflammation in the body,which is involved in the pathogenesis of PCOS. |
PDF Full Text Request