Effect Of Liquid Sucrose Consumption On Digestion And Metabolism In Normal And Fat Mice

Obesity is an increasingly serious global public health problem.Its harm is not only manifested in weight gain,but also leads to the prevalence of metabolic diseases such as diabetes and hypertension.The intake of sugar is one of the main causes of obesity,and the intake of liquid sugar with the same calories is more likely to lead to obesity than that of solid sugar,but its digestion and metabolism mechanism is still unclear.The purpose of this study was to investigate the changes in digestion and metabolic physiology of naturally obese and normal weight individuals after ingesting sucrose solutions of different concentrations,and whether the differences were significant.40 obese and 40 normal adult female Kunming mice were fed with 0,20,40 and 60%sucrose water for 30 days,respectively.During this period,blood glucose was measured every 15 days,body weight was weighed every 7 days,and the food intake of the mice for 24 hours was weighed and calculated.Thirty days later,blood samples were dissected and collected to detect glucose,triglyceride,urea nitrogen,uric acid,insulin,glucagon,glucagon-like peptide-1,leptin,ghrelin,adiponectin and other indexes.The liver was frozen to detect FGF-21,liver glycogen,glycogen synthase,glycogen phosphorylase and other indexes.The activities of sucrase,maltase and aminopeptidase were detected by cryopreservation of small intestine.The results showed that in terms of digestion,the food intake of fat and normal weight mice decreased by 42%and 46%with the increase of sucrose concentration,the digestibility of fat mice also decreased by 5%,and the digestibility of fat mice in 20%and 40%groups was significantly lower than that in normal weight group,but the body weight of the two groups decreased without significant difference.Two groups of sucrase and maltase activity of small intestine increased significantly with sucrose concentration of 80%and 60%,ammonia peptide enzyme activity is reduced by 30%.In terms of metabolism,with the increase of sucrose concentration,the serum triglyceride concentration of fat and normal weight mice had no significant change.The contents of urea nitrogen and uric acid in the two groups were significantly decreased by about 35%,which might be caused by the increase of sucrose intake and the decrease of protein intake.The levels of insulin,glucagon-like peptide-1,leptin,ghrelin and adiponectin in normal group were significantly increased,and the secretion of insulin and leptin in 60%group was nearly 3 times higher than that in control group.However,there was no such change in the fat group,but the contents of insulin,glucagon-like peptide-1,leptin,ghrelin and adiponectin in the fat group were significantly higher than those in the normal group when the mice were fed 20 and 40%sucrose solution.Because the levels of insulin,glucagon-like peptide-15 leptin and other hormones in serum of fat mice were higher than those of normal body weight mice,the body was less sensitive to the metabolic regulation after the diet supplemented with high concentration of sucrose than that of normal body weight mice.The contents of FGF21,liver glycogen,glycogen synthase and glycogen phosphorylase in liver tissue were significantly positively correlated with the concentration of sucrose solution in the fat group.Compared with the control group,glycogen in total increased by 86%,per wet tissue increased by 78.6%,glycogen synthase in total increased by 38%,per wet tissue increased by 22.6%,and glycogen phosphorylase in total and per wet tissue increased by 19%in 60%group,but no such changes were observed in normal weight group.In terms of digestion,because of the two groups of mice were obtained from sucrose solution sugar intake increased,and the intake of solid food reduce lead to the reduction of protein intake,cause the corresponding changes in digestive enzyme activity,further verify the "adaptive modulation hypothesis".Metabolic ways,fat mice on the diet of high concentration of sucrose metabolism regulation is not sensitive,so the accumulation of hepatic glycogen,hepatic glycogen synthesis decomposition enzyme levels increased significantly,the normal mice sucrose increased metabolic regulation is more sensitive to the diet,the excessive intake of sucrose metabolism,timely not cause excessive accumulation of hepatic glycogen..