Clinical Research Of Erythrocyte Sedimentation Rate、C-reactive Protein And Serum Cytokines In Pulmonary Infection

Objective : This research investigates the clinical value of erythrocyte sedimentation rate(ESR)、C-reactive protein(CRP)and serum cytokines in the diagnosis and severity judgment of pulmonary infection.In addition,the mouse inflammatory model was built to explore the possible mechanism of cytokines affecting inflammatory response,so as to provide reference for early effective treatment and prognosis.Methods: Patients with severe pulmonary infection were selected as study group 1and further divided into survival group and death group.Pulmonary infection patients admitted to our hospital at the same time were selected as the study group 2,and healthy subjects admitted to our hospital at the same time were selected as the control group.The levels of ESR CRP,IL-17 A and IL-10 were tested.Mouse Ana-1macrophage inflammatory model was established by LPS.The m RNA and protein expression levels of My D88 and NF-κB in LPS group and LPS+ IL-4 group were detected by both quantitative PCR and Western blot,respectively.The nuclear/cytoplasmic ratio,IL-6 and TNF-α both in LPS group and LPS+ IL-4 group were detected by ELISA.Results:1.The levels of ESR,CRP and IL-17 A in study group 1 were higher than those in both study group 2 and control group(P<0.05).However,the level of IL-10 was lower than that in both study group 2 and control group(P < 0.05).The level of ESR,CRP and IL-17 A in the survival group showed a decreasing trend over time,while in the mortality group,ESR,CRP and IL-17 A levels showed an increasing trend(P <0.05);the level of IL-10 in the survival group showed an increasing trend over time,while that in the mortality group showed a decreasing trend with time(P < 0.05).2.In the LPS group,the m RNA and protein expression levels of My D88 and NF-κB showed an increasing trend over time(P < 0.05).However,the m RNA expression level of My D88 in LPS+IL-4 group didn’t change(P > 0.05),while the protein expression level increased first and then decreased.The expression of NF-κB in LPS+IL-4 group showed an obvious increase over time(P < 0.05),but there was no statistical difference with that in LPS group(P>0.05).Conclusion: Dynamic levels of ESR,CRP,IL-17 A and IL-10 can be used as important diagnostic and prognostic indicators for pulmonary infection.Among them,ESR,CRP and IL-17 A were positively correlated with the severity of pulmonary infection,while IL-10 was negatively correlated with the severity of pulmonary infection.IL-4 can down-regulate the expression of cytokine IL-6 and TNF-α.We hypothesize that the anti-inflammatory effect of IL-4 is related to inhibiting the activation of My D88-NF-κB signaling pathway.