Epidemiological Survey On Sheep Mycoplasma Pneumoniae And Isolation,Identification Of Mycoplasma Ovipneumoniae From Jilin Province

Mycoplasma ovipneumoniae(MO)can cause pneumonia in wild animals such as sheep,goats and bighorn sheep,and sheep are most sensitive to this pathogen.Sick sheep usually have symptoms such as high fever,wheezing,coughing,and getting thinner.The current treatments are mainly based on drug treatment,but the disease spreads very fast,has strong regional transmission characteristics,and is easier to spread in rainy and humid environments.The infection rate of this disease has been increasing year by year in the national sheep flock,which seriously disturbs the healthy development of the sheep industry.In the first part,PCR detection method was used to detect MO in the northwest,west,south and east of Jilin Province and analyzed risk factors.The results showed that the total positive rates of Wohu,Yongjia,Changling,Longjing,Liuhe,Yongji,Taonan were 35.36%,33.33%,47.05%,21.73%,14.28%,21.42% and 44.11%.By SPSS statistical analysis found that,in this study the age,season and region mycoplasma infection is a significant factor in the western region of Jilin Province MO trend is relatively serious.By 16 S r RNA sequencing of positive samples,some representative sequences were selected to construct a phylogenetic tree.The results showed that it was divided into three branches.The representative sequences are on the same branch as other sequences of the MO genus,but are different from most domestic isolates.Belong to the same branch.In the second part of this study,MO isolation and culture were carried out on the lung and nasal swabs of dead sheep from suspected MO infection in Jilin Province.The disease material at the border between disease and health was taken,treated and inoculated into Mycoplasma medium(non-poultry source)for isolation and culture,and finally 2 strains of suspected MO were isolated from 4 sheep lungs.After PCR detection and sequencing,the sequence was compared with the known sequence published in Genbank for Blast comparison analysis,and the isolate was further determined to be MO from the molecular level.In order to understand the growth of the isolates in different media,the color change unit(CCU)counting method was used to compare the growth of the isolates in the media.The results showed that the growth rate of KM2 was 108ccu/ml,and the Mycoplasma medium(non-avian origin)was 106ccu/ml,indicating that the isolates grew faster in KM2 medium.In this paper,some areas of Jilin sheep MO epidemiological survey,carried out the infection risk factor analysis,and separated into two MO.These research results provide a scientific basis for the prevention and control of MO in Jilin Province,and also provide a useful reference for the development of MO vaccines in the future.