| Purpose: In this experiment,through the literature analysis of arteriosclerosis obliterans(ASO)syndrome,ID name,specification of symptoms,combining the "card" and the card is to carry out symptoms,arterial occlusive disease of TCM in the forward-looking specification;clear ASO TCM research foundation,further through Quyu Huazhuo decoction treatment.The endothelial injury theory as a starting point,clear Quyu Huazhuo efficacy and mechanism,in order to provide theoretical and experimental basis for the clinical treatment of ASO syndrome.Material and method: 1.based on the "TCM syndrome diagnostic efficacy standards","Chinese and Western medicine diagnosis and treatment of peripheral vascular disease," textbook "Chinese medicine surgery"(respectively called Li Ben He Ben,Yang,Gu Ben Lu Ben),"Chinese and Western medicine combined with surgery"(referred respectively to the Meng and where the the Wei,Chen Ben,Li Ben)and other authoritative literature comparing ASO of TCM,and searching for nearly 20 years,ASO of TCM from CNKI,Wan Fang database Chinese journal literature,selected TCM clear 61 references of the actual situation of study in ASO clinical diagnosis.2.the experimental animals were studied by male white rabbits.The male white rabbits were unified in the animal experiment center of the Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine(20~26 ℃,the humidity was 40%~70%)Feed.They were randomly divided into five groups: blank group,model group,Chinese medicine positive group(Zhongyang group),high dose group of removing stasis and turbid turbid,and low dose group of removing blood stasis and turbidity,with ten rats in each group.Blank group: normal diet,free drinking water;model group: high fat diet(87.5% basic feed +10% lard +0.5% cholic acid +2% cholesterol),after a week of adaptive feeding,injection of bovine serum albumin(BSA),230 mg / kg,a week after the re injection of BSA,125 mg per kilogram of body weight for 4 weeks.After high fat diet,the balloon(3.0mm)male rabbits right carotid artery injury surgery,given high fat diet to 8 weeks,from Zhongyang;group: on the basis of the model group with Xuezhikang orally,1 times a day,for 4 consecutive weeks,free drinking water;removing stasis Huazhuo Decoction high and low dose group were orally,1 times a day,for 4 consecutive weeks,free drinking water.After treatment,male rabbits were anesthetized and blood was taken from abdominal aorta.Blood was collected to detect blood lipids,homocysteine and C reactive protein.Blood and hemorheology were collected from whole blood and plasma.HE staining was used to observe pathological changes of common carotid artery endothelial tissue.Results: 1.the standard literature ASO is different from each other,and the difference is great.There are different similarities and differences between various documents and different versions of the same kind of literature.In other words,there are many standards of ASO syndrome differentiation,but there is no unified standard.2.ASO’s clinical syndrome differentiation can be roughly divided into 3 categories.The first one is to fully comply with the authoritative literature;the second one is to adapt to the authority literature;the third is the new evidence that is not related to the authoritative literature and flexibly launched.3.Is a clinical diagnosis of the syndrome differentiation standard is not unified,the two is arbitrary,each one takes what he needs as a diagnostic standard;and no binding requirements,must follow the self can go its own way,introduce new evidence,it is unthinkable in modern medicine;the three is the highly discrete diversity of ASO contributed to the clinical diagnosis standard the result of clinical syndrome differentiation,is subjective and arbitrary situation.4.And two of their respective symptoms of contradiction,relationship between syndromes and their symptoms are inconsistent,resulting in limit card between the confusion;symptom presentation is not standardized,overlapping is common;a large number of symptoms,especially with disease related symptoms of mixed.The other cases of ASO are generally the same,so it is not redundant.These problems all reduce the level of ASO clinical diagnosis and diagnosis,and then affect the recognition of clinical efficacy,and the application of TCM diagnosis and treatment experience. 5.model group compared with the control group,the total cholesterol,triglyceride,high density lipoprotein,low density lipoprotein,were significantly increased;blood stasis Huazhuo Decoction low dose group(4.20 g crude drug /kg),Quyu Huazhuo high dose group(8.40 g crude drug /kg)compared with the model group,triglyceride and low density lipoprotein content compared were significantly decreased;high dose group(8.40 g crude drug /kg)high density lipoprotein content increased significantly compared(P < 0.05).6.model group compared with the control group,the serum C reactive protein and homocysteine were significantly increased;Quyu Huazhuo Decoction low dose group(4.20 g crude drug /kg),Quyu Huazhuo high dose group(8.40 g crude drug /kg)compared with the model group,with the decrease of C reactive protein content;the high dose group(8.40 g crude drug /kg)homocysteine concentrations compared significantly decreased(P < 0.05).7.model group compared with the control group,plasma viscosity,erythrocyte aggregation index,whole blood high,middle and low shear increased significantly,removing stasis turbid and low dose group(4.20 g crude drug /kg),Quyu Huazhuo high dose group(8.40 g crude drug /kg)compared with the model group,plasma viscosity,red cell aggregation index,whole blood high,middle and low shear decreased significantly(P < 0.05).8.groups of common carotid artery wall smooth,normal morphology,endometrial thickening,no obvious lesion;model group with vascular fibrous plaque,local tissue degeneration and the emergence of large areas of calcification,compression and atrophy of tubular smooth muscle thinning,adventitia angiogenesis and connective tissue proliferation and infiltration of lymphocytes and plasma cells;foam cells visible range the Zhongyang group under artery endothelial aggregation;low dose group showed a large amount of fibrous plaque irregular hyperplasia,foam cell degeneration;high dose group showed a small amount of fibrous hyperplasia of plaque.Conclusion: 1.it is found that there are demonstrative problems in TCM syndrome differentiation between authoritative documents,academic circles,authoritative literature and academia,and the basic ideas and methods of syndrome differentiation are put forward.2.the decoction of removing stasis and turning turbid has a clear effect on reducing blood lipid in ASO white rabbits.3.the decoction of removing stasis and turning turbid has a clear effect on the reduction of homocysteine in ASO white rabbits.4.the prescription of removing stasis and transforming turbidity has a clear effect on reducing the C reactive protein in ASO white rabbits.5.the decoction of removing stasis and turning turbid has a definite effect on improving the hemorheology of ASO white rabbits.6.the decoction of removing stasis and turning turbid can improve the change of Pathomorphology in ASO white rabbits. |
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