Mechanism Of Acupuncture Treatment For Menopausal Syndrome With Deficiency Of Kidney Yin Based On Brain-Gut Axis

Research purpose: Based on the theory of "brain-gut axis",this study explores the relationship between the intestinal flora and 5-hydroxytryptamine(5-HT)levels in patients with menopausal syndrome and anxiety disorders,and explores the comprehensive treatment of menopause with acupuncture The mechanism of symptom with anxiety disorder provides a theoretical basis for clinical treatment of menopausal syndrome with anxiety disorder.Research method: Select 20 patients with kidney-yin deficiency type menopausal syndrome with anxiety disorder that meet the criteria of intake and discharge,and use the "shen-yishen" acupuncture method for electroacupuncture treatment,using self-control before and after,through improved kupperman score,tidal Thermal score,Hamilton anxiety scale(HAMA),and TCM syndrome score were used to evaluate the efficacy.Ten healthy women aged 40-60 were selected as the control group,and the ELISA method and 16 S r DNA high-throughput sequencing method were used to detect the serum 5-HT levels and the species diversity composition of the intestinal flora in the control group and the test group before and after treatment.And the abundance was compared with the test group before treatment,and SPSS22.0 was used for statistics to draw conclusions.Research result:1.Comparison of the experimental group before and after treatment,the modified kupperman score decreased after treatment,and the difference was statistically significant by t test(P<0.05);HAMA scale,TCM syndrome score,hot flash score decreased,and the difference was found by rank sum test It is statistically significant(P<0.05),suggesting that acupuncture can improve the clinical symptoms of patients with menopausal syndrome and anxiety disorder.2.In terms of the species abundance and diversity of the intestinal flora,the test group is lower than the control group,and the test group is higher after treatment than before treatment;at various levels,the test group is compared before and after treatment,at the phylum level,After treatment,Firmicutes(Firmicutes)decreased,Bacteroidetes(Bacteroides)increased,at the class level,Clostridia(Clostridia)after treatment decreased,Bacteroida(Bacteroides)increased,at the mesh level,Clostridiales(Clostridiales)decreased after treatment,Bacteroidales(Bacteroides)increased,at the family level,Lachnospiraceae(Laterospiraceae),Bacteroidaceae(Bacteroidaceae)after treatment increased,at the genus level After treatment,the proportions of Bacteroides and Faecalibacterium increased;the two groups were compared,the test group was at the phylum level,and the proportion of Bacteroides and Proteobacteria was relatively small.At the level,the test group has relatively few Bacteroides and Proteobacteria.At the family level,the dominant bacteria in the test group is Lachnospiraceae,while the dominant bacteria in the control group is Ruminococcaceae.After the rank sum test,the comparison of the test group before and after treatment,and the comparison of the two groups in the analysis of the difference in α diversity between the groups were statistically significant(P<0.05);the difference in the analysis of the difference in β diversity between the test groups before and after treatment was not statistically significant(P >0.05),the difference in the analysis of the difference of β diversity between the two groups was statistically significant(P<0.05).The analysis of differences between groups was carried out by molecular analysis of variance(Amova).The differences between the two groups before and after treatment in the experimental group were statistically significant(P<0.05).3.The 5-HT level of the experimental group before treatment was higher than that of the control group,and the 5-HT level of the experimental group decreased after treatment.The difference was statistically significant by t test(P<0.05).Analysis conclusion:1.Acupuncture can change the species abundance and diversity of intestinal flora in patients with kidney yin deficiency type menopausal syndrome with anxiety disorders.2.Acupuncture can reduce the serum 5-HT levels of patients with kidney-yin deficiency type menopausal syndrome with anxiety disorders.3.Acupuncture treatment has a good clinical effect on patients with kidney yin deficiency type menopausal syndrome with anxiety disorder.It may be that the intestinal flora affects the brain nerve function through the enteric nervous system,improves the serum 5-HT level,and produces anti-anxiety effects.It is the hub of the brain-gut axis.