Effects Of Blueberry On Bone Marrow Hematopoiesis And Liver KLF11、 Spleen GATA1 Two Hematopoietic Factors In Carbon Tetrachloride-induced Liver Fibrosis Model

Objective:To observe the effects of blueberry on the expression of GATA1 protein&m RNA in spleen,the KLF11 protein&m RNA protein in liver and bone marrow hematopoietic status,which in the model of rats with liver fibrosis,explore its possible mechanism.Methods:Forty healthy rats of the same age were randomly divided into normal control group,blueberry control group,model group and blueberry intervention group with 10 rats in each group.We established liver Fibrosis Model Rats with carbon tetrachloride（CCl₄）.The ALT,AST and Four items for liver fibrosis（IV-C,PC III,HA,LN）of serum were measured in each group.The liver tissue was examined by pathological examination with HE&Masson Staining.Western-blot was used to detect the protein expression of KLF11,α-SMA and COAⅠin liver and the protein expression of GATA1 in spleen.Detection of m RNA expression of KLF11,COAⅠin liver tissue by Real-time PCR.The proportion and morphological changes of hematopoietic cells in rat bone marrow were counted by manual classification and observed.The peripheral blood cells were counted by instrument and the proportion and morphological changes were counted by manual classification.Results:1.Results of liver fibrosis model in rats:The results of pathological examination of liver tissue showed that the liver tissue of the model group showed typical characteristics of liver fibrosis and the grade of liver fibrosis was significantly higher than that of the normal control group;Compared with other groups,the expression of COAI m RNA,COAI protein,α-SMA protein in liver tissue and the serum ALT、AST、HA、PCⅢof model group in rats were significantly increased（P<0.01）,IV-C,LN could not be detected by unknown reason;Compared with the model group,the above indexes of liver fibrosis in blueberry intervention group were significantly improved.2.Liver hematopoiesis related results:the expression of KLF11 protein&m RNA in liver tissue was significantly decreased in the model group（P<0.01）than that in the other groups;3.Spleen hematopoiesis related results:Compared with other groups,the expression of GATA-1 protein&m RNA in the spleen was increased in the model group（P<0.01）.4.Results of the morphological examination of peripheral blood and bone marrow cells:in blood,compared with other groups,there were more nucleated erythrocytes in the peripheral blood in the model group（P<0.05）,and the proportion of target erythrocytes was increased in the model group（P<0.05）.IN BONE MARROW,Compared with other groups,the proportion of bone marrow immature red blood cells was increased in the model group（P<0.05）,which was mainly in the Orthochromatic normoblasts and Ploychromatic normoblasts.Conclusion:1.intraperitoneal injection of carbon tetrachloride in rat model of liver fibrosis successfully,using blueberry intervention can improve liver fibrosis;2.During liver fibrosis induced by carbon tetrachloride,the KLF11 of liver decreased,which shows that the hematopoietic function may decreased,while high expression of GATA1 in spleen may lead to extramedullary hematopoiesis.Hepatic fibrosis may affect red blood system hematopoiesis in bone marrow,which is characterized by proliferation of bone marrow erythrocytes,peripheral blood target erythrocytosis and nuclear erythrocytosis.3.Using blueberry to improve carbon tetrachloride-induced liver fibrosis can effectively alleviate the effects on liver,spleen and bone marrow hematopoiesis,including reducing its effects on bone marrow and erythroid cells in the blood,increasing the expression of intrahepatic KLF11 and reducing the expression of GATA1 in the spleen.