Protective Effects Of Blueberry Extract Against Acute Alcohol-induced Liver Injury

Drinking large quantities of alcohol and alcoholic beverages will lead to alcoholic liver damage (ALD) of the place, as China's rapid economic development and improve people's quality of life ever, the incidence of alcoholic liver disease have shown a rising trend. Phytochemicals on human health function is currently a hot topic in nutrition research, one present in all natural vegetables and fruits. Blueberry is one of higher nutritional value of fruits, contain a variety of bioactive substances, which is a kind of anthocyanin. On the basis of animal experiments in this study was to observe the blueberry extract on acute alcoholic liver injury in rats.Object:In this study, acute alcoholic liver injury in mice as a model in mice by reduced glutathione, malondialdehyde, the determination of triglycerides, and mouse liver biopsyand other factors research and analysis To explore the blueberry extract on alcoholicliver injury in rats.Method:1,Animals:60 male Kunming mice, body weight (28±2) g.2,Animal and dose group:Randomly divided into normal control group, ethanol model group, positive control group(30mg/kg),blueberry extract the low dose group(30mg/kg), blueberry extract high-dose group(60mg/kg),5groups of 10～12.3,Experimental methods:During the experiment, the low-dose group of blueberry extract, the high-dose group of blueberry extract, and positive control mice given test substance by gavage, the control group and the liver injury group received distilled water for 30 days. Weighed once every 3-4 days, weight adjusted according to the amount fed.31 days trial, In addition to the normal control group, other groups were diluted with ethanol solution (concentration 50%) caused liver damage, a gavage 12-14ml/kgBW(equivalent dose of ethanol in 6000-7000mg/kgBW). After fasting for 12 hours, mouse eye blood with heparin, mice were killed and the liver weight, and then right side of the liver caudate lobe,10% formalinfixed. The liver, made of 10% homogenate.4,Target detectionMouse plasma and liver glutathione determination, with reference to the Ministry of Health, "health food inspection and evaluation of technical specifications" 2003Edition,according to a method as "the chemical liver injury secondary protection". Mouse liver and brain malondialdehyde detection method with reference to "health toxicology". Formalin fixed liver tissue after dehydration,embedded in paraffin,sliced,fishing films,hematoxylin and eosin staining, observed under the microscope and analyzed.5,Data processing:Results indicated that the data x±s,using statistical software SPSS 13.0,using single factor analysis of variance compared differences between groups, application LSD pair wise comparison method.Result:Mice in each group beginning and end of the experiment body weight and weight gain were not significantly different.Alcoholic liver TG model group was significantly higher than the normal control group. Blueberry extract high dose animal model of liver TG content was lower than the control group, close to the positive control group.Ethanol model group, liver MDA were significantly increased. The tested drugs liver MDA was significantly lower than ethanolmodel group. The test substances in the brain of mice alcohol MDA content was lower than the model group, but the difference was not statistically significant.Ethanol model group, liver glutathione levels were significantlyreduced. Blueberry extract high dose levels of liver glutathione in model group than alcohol, with the positive control group (grape seed extract) close. Alcohol model mice whole blood reduced glutathione were significantly lower than the control group, blueberry extract low and high dose group were higher than ethanol model group.Ethanol model group Liver index increased in mice. Blueberry extract low and high dose groups were lower than the alcohol, the liver index model group, with the positive control group (grape seed extract) close. Ethanol model group, the liver in mice liver steatosis. And each test substance the liver of mice with different levels reduced.Conclusion:Blueberry extract with alcoholic liver injury in mice can reduce lipid peroxidation, antioxidant system to help the body eliminate free radicals, reduce liver fat accumulation, reducing the pathological changes of liver tissue.The results suggest that blueberry extract on alcoholic liver injury in some rats.