Relationship Between Serum Sclerostin And Abdominal Aortic Calcification In Maintenance Hemodialysis Patients

Objective The purpose of this article is to study the relationship between serum sclerostin and abdominal aortic calcification in maintenance hemodialysis patients,and to analyze the influencing factors related to abdominal aortic calcification.It can provide further ideas for diagnosing and preventing vascular calcification in patients with maintenance hemodialysis.Methods From November 2018 to August 2019,80 patients with underwent maintenance hemodialysis in the Department of Nephrology of the First Affiliated Hospital of Shihezi University were selected as the experimental group,and 60 healthy people in the same period were selected as the control group.Collect the general information of the patient,and the laboratory of our hospital will test the blood calcium,blood phosphorus,blood magnesium parathyroid hormone and other related biochemical indicators.All the subjects were assessed with abdominal aortic calcification score according to the kauppila semi-quantitative integration method.The experimental group(before hemodialysis)and the control group was early in the morning,5ml of cubital vein blood was collected on an empty stomach,which was left at room temperature for 2 hours or overnight at 4 ℃.After centrifugation at 1000 rpm for 20 minutes,the supernatant was placed in a cryopreservation tube and stored in a refrigerator at-80 ℃.After the samples were collected and thawed uniformly,the serum sclerostin levels was measured by ELISA.Analyze the general condition,biochemical indexes,serum sclerostin levels and calcification of abdominal aorta in experimental group and control group;and divide the experimental group into non-calcification group,mild group,and severe group according to the calcification of the abdominal aorta;analyze the relationship between the serum sclerostin levels and clinical biochemical indicators serum among the three groups.;analyze the correlation between serum sclerostin level and abdominal aortic calcification score,the correlation between abdominal aortic calcification score and related biochemical indicators;and the use of multi-factor Logistic regression to analyze the influencing factors of abdominal aortic calcification.Results(1)Compared with the healthy control group,serum sclerostin levels in patients with maintenance hemodialysis were significantly increased(P<0.05);(2)Among 80 patients with maintenance hemodialysis,the average age was(58.8±15.20)years,the average dialysis age was(35.03±29.74)months,and 58 patients had abdominal aortic calcification(72.5%).(3)In the MHD group,the difference in sclerostin levels among the three groups was statistically significant(H=19.705,P<0.001).Among them,the serum sclerostin in the mild group and the moderate and severe group was higher than that in the non-calcification group,and the serum sclerostin in the moderate and severe group was higher than that in the mild group.Differences in age(F=43.33,P=0.006),dialysis age(H=33.429,P<0.001),PTH(H=9.568,P=0.008),P(F=7.862,P=0.001)among the three groups significance in science;the dialysis age,P,PTH in the mild group were higher than that in the non-calcification group;the age,dialysis age,PTH,P in the moderate and severe group were higher than the the non-calcification group,and the dialysis age in the moderate and severe group was higher than the mild group.(4)According to Spearman correlation analysis,the serumsclerostin level was positively correlated with abdominal aortic calcification score(r=0.512,P<0.001);abdominal aortic calcification score was correlated with age(r=0.371,P=0.001)and age of dialysis(r=0.535,P<0.001),parathyroid hormone(r=0.358,P=0.001),and phosphorus(r=0.29,P=0.003)were positively correlated.(5)Multi-factor Logistic regression showed age(OR=1.113,95%CI=1.045~1.185,P=0.001),age of dialysis(OR=1.053,95%CI=1.022~1.086,P=0.001),blood phosphorus(OR=5.079,95%CI=1.108~23.28,P=0.036),serum sclerostin(OR=1.034,95%CI=1.008~1.061,P=0.011)are independent risk factors for abdominal aortic calcification.Conclusion In maintenance hemodialysis patients,the incidence of abdominal aortic calcification is relatively high.The level of serum sclerostin is positively correlated with abdominal aortic calcification,which is a risk factor for abdominal aortic calcification and is of great significance for the prediction of abdominal aortic calcification.