Effects Of MAP Solution And MEI Solution On The Exposure Rate Of PS On Membrane Of The RBCs And The Phagocytic Rate Of RBCs By Monocytes In Dogs

Objective: To observe the effect of MAP solution and MEI solution on the exposure rate of PS on membrane of the RBCs and the phagocytic rate of RBCs by monocytes in dogs.Methods: Thirty healthy adult dogs(10-15 kg)were randomly divided into three groups:control group(group NS,n=10),Multiple-Electrolytes-Injection solution group(groupMEI,n=10)and Mannitol-adenine-phosphate solution group(group MAP,n=10).400 ml blood was losing slowly through femoral vein and the blood was salvaged,The washing fluid in the group NS was 0.9% nomal saline,while the group MEI was Multiple-Electrolytes-Injection solution and the group MAP was Mannitol-adenine-phosphate solution.Then the Red blood cell salvaged(RBCs)were directly labeled with fluorescein isothiocyanate(FITC)as a probe and infused return to the dogs.The venous blood was taken from the dog of three groups before blood salvaged to detect the ATP content in the RBC,and the ATP content in RBCs was detected immediately after blood salvaged.The blood was taken at 24 h,48h,72 h after the RBCs being infused return,and the phosphatidylserine(PS)exposion on membrane of FITC-RBCs were detected by flow cytometry,the spleen was taken to detect the phagocytic rate of monocytes engulfing FITC-RBCs after 72 h FITC-RBCs reinfused return to the dogs in both gruops.Results: 1.The ATP content in venous RBC and RBCs in three groups: The ATP content in venous RBC before IBS in three groups.There was no statistical difference in the ATP content in venous RBC in three groups(P> 0.05).Compared the ATP content in RBCs with the ATP content in venous RBC in each group.The ATP content in RBCs in the NS group and the MEI group were lower than that in venous RBC(P<0.05),and the ATP content in RBCs in the MAP group was significantly higher than that in venous RBC(P<0.05).The comparison of the ATP content in RBCs after IBS in three groups.The ATP content in RBCs in the MAP group was significantly higher than that in the NS and the MEI groups(P < 0.05).There was no significant difference between the ATP content of RBCs in the NS group and the MEI group(P>0.05).2.The PS exposure rate on membrane of the RBCs in three groups of dogs: As the infusion time extension,The exposure rate of PS on membrane of the RBCs in the three groups was increased.The exposure rate of PS on membrane of the RBCs in the NS group was higher than that in the MEI group and the MAP group when the FITC-RBCs were returned to animals at 24 hours,48 hours,and 72 hours(P <0.001).The exposure rate of PS on membrane of the RBCs in the MEI group was higher than that in the MAP group when the FITC-RBCs were returned to animals at 24 hours(P=0.02).There was no statistical difference between the exposure rate of PS on membrane of RBCs in the MEI group and that in the MAP group when the FITC-RBCs were returned to animals at 48 h and 72h(P>0.05).3.The phagocytic rate of monocytes in three groups after RBCs infusion into dogs within 72 hours: The phagocytic rate of monocytes in the MEI group and in the MAP group were lower than that in the NS group(P <0.05).There was no statistical difference between the phagocytic rate of monocytes in the MEI group and that in the MAP group(P> 0.05).Conclusion: 1.when the NS solution was used as the the blood recovery washing solution,the ATP content in RBCs was significantly reduced.When the MEI was used as the washing solution,the ATP content in RBCs was also significantly reduced.But when the MAP solution was used as the washing solution could improve the ATP content in RBCs;2.When the MAP solution or the MEI solution was used as the blood recovery washing solution,the PS exposure rate on membrane of the RBCs could be significantly reduced.3.The MAP solution or the MEI solution was used as the blood recovery washing solution could reduce the phagocytosis rate of RBCs by spleen monocytes and extend the survival time of the RBCs in the body.