Correlation Analysis Between KIN17 Protein Expression With The Clinical Features/Prognosis Of Epithelial Ovarian Cancer

Objective:To observe the relationship between the expression of DNA damage repair-related KIN17 protein and the clinical features/prognosis of epithelial ovarian cancer(EOC).Methods:1.Oncomine database and Kaplan-Meier database were employed to analyze the correlation between the DNA/mRNA level of KIN17 and the development/prognosis of ovarian cancer.2.A total of 56 paraffin-embedded EOC specimens were obtained from the patients diagnosed and treated in the Second Hospital of Jilin University between January 1,2012 and January 31,2018.The other 10 EOC specimens and their own adjacent tissues were collected for Western Blot,and these cases were diagnosed and treated in the Second Hospital of Jilin University from February 1,2018 to August 31,2019.3.Western Blot was used to detect the expression of KIN17 protein in EOC tissues and their own adjacent tissues.IHC was used to evaluate the expression of KIN17protein in the paraffin-embedded EOC specimens.4.Statistical analyses were performed with SPSS 23.0 software.T tests were used for comparison of KIN17 protein in EOC tissues and adjacent tissues.The correlation between KIN17 protein expression and clinical features was evaluated by X~2 test.Overall survival(OS)curves were plotted by Kaplan-Meier method.The relationship between overall survival and expression of KIN17 protein as well as the clinical features of EOC were compared by the log-rank test.The independent risk factors for OS were assessed by the COX regression.Results:1.The results obtained from the Oncomine database showed that the DNA and mRNA level of KIN17 were significantly(P<0.05)up-regulated in ovarian serous adenocarcinoma compared with normal ovarian cancer.Moreover,compared with borderline ovarian surface epithelial-stroma tumor,the mRNA level of KIN17 was significantly obviously(P<0.01)up-regulated in ovarian cancer.Additionally,the results obtained from Kaplan-Meier database showed that the patients with high KIN17mRNA level had shorter OS than the ones with low KIN17 mRNA level.2.Compared with the adjacent tissues,the expression of KIN17 protein was remarkably(P<0.01)increased in EOC tissues.3.The results of IHC showed that high KIN17 protein expression was significantly(P<0.05)correlated with lymphatic metastasis.No significant correlation was found between the KIN17 protein expression and age,stage,ascites volume,the preoperative level of CA125,the preoperative level of HE4,tumor size,EOC type and pathological grade.4.Univariate survival analysis showed that compared to EOC patients in early stage(stage I+II),OS of the EOC patients in advanced stage(stage III+IV)was significantly(P<0.01)lower.The OS of EOC patients in type II EOC group was significantly(P<0.05)decreased than that in type I EOC group.The patients identified as high grade had a shorter OS than the patients with low grade(P<0.01).The patients suffered from lymph node-positive had a poorer OS than the one without lymph node-positive(P<0.05).Additionally,OS of EOC patients in high KIN17 expression group was markedly(P<0.05)shorter than that in low KIN17 expression group.No significant correlation was found between OS and age,tumor size,ascites volume,the preoperative level of CA125,the preoperative level of HE4 and the expression location of KIN17 protein.Subsequently,multivariate analysis found that high KIN17 expression and advanced stage(stage III+IV)were independent risk factors for the OS of EOC patients.Conclusions:1.The DNA,mRNA and protein levels of KIN17 are closely related to the initiate and development of EOC.The high level of KIN17 mRNA is a risk factor affecting the OS of ovarian cancer patients,and the high KIN17 protein expression is an independent risk factor affecting the OS of EOC patients.2.High KIN17 protein expression is associated with positive lymph node metastasis in EOC patients.3.Advanced stage(stage III+IV),high grade,type II and positive lymph node metastasis are risk factors affecting the OS of EOC patients.4.High KIN17 protein expression and advanced stage(stage III+IV)were independent risk factors affecting the OS of EOC patient...