Expression Of HOXB9 In Colorectal Carcinoma Tissues And Its Relationship With Micro-angiogenesis

Objective: To study the expression of homeobox gene B9(HOXB9)in colorectal carcinoma tissues;To analyze the relationship between HOXB9 and clinicopathological factors in colorectal carcinoma tissues;To explore the correlation of HOXB9,and VEGF and micro-angiogenesis in colorectal carcinoma tissues;To provide a preliminary basis for HOXB9 to be a biomarker of anti-angiogenesis targeted therapy and to judge the therapeutic effect of bevacizumab.Methods: The expression of HOXB9,CD34,and VEGF in 156 cases of colorectal carcinoma tissues and normal mucosal tissues were detected by the standard streptavidin-peroxidase(SP)technique.And tumor microvessel density was measured by labeling CD34 to reflect tumor micro-angiogenesis.The SPSS 21.0 software was used to analyze the expression of HOXB9 in colorectal carcinoma tissues and its normal mucosa tissues.And to study the relationship between the expression of HOXB9 and the clinicopathological features of colorectal cancer.And to explore the correlation between HOXB9 and VEGF and micro-angiogenesis.Results: 1.HOXB9 is highly expressed in colorectal carcinoma tissues,the expression rate of HOXB9 was 62.18%(97/156),and it was significantly higher than the positive expression rate of normal mucosa 5.13%(8/156).The difference between the two groups was statistically significant(P<0.05);The expression rate of VEGF was 67.31%(105/156),and the expression rate in normal mucosa was 8.33%(13/156).The difference was statistically significant(P<0.05).The values of CD34-MVD in colorectal carcinoma tissues and normal mucosa tissues were 16.04±3.23、5.75±1.94,the difference was statistically significant(P<0.05).2.In colorectal carcinoma tissues,the expression rate of HOXB9 increased with the depth of tumor invasion.When the depth of invasion reached T1,T2,T3 and T4,the expression rates were 41.67%(5 / 12),52.8%(28 / 53),60.34%(35 / 58)and 87.88%(29 / 33).The expression rate of T4 was significantly higher than that of T1,T2 and T3,P<0.05.There was no significant difference between T1,T2 and T3.The expression rate of HOXB9 was 84.71%(72 / 85)in colorectal carcinoma tissues with positive lymph node metastasis.That was significantly higher than the expression rate of lymph node negative carcinoma tissues(35.21%),P<0.05.The expression rates of HOXB9 were 32.00%(8 / 25),36.96%(17 / 46)and 84.71%(72 / 85)in colorectal carcinoma tissues with TNM stageⅠ,Ⅱand Ⅲ,respectively.The expression rate of HOXB9 in stageⅠandⅡwas significantly lower than that in stageⅢ.P<0.05.3.HOXB9 and VEGF were expressed in different degrees in colorectal carcinoma tissues.In HOXB9-positive colorectal carcinoma tissues,the positive expression rate of VEGF was 76.29%(74/97).In colorectal carcinoma tissues with negative HOXB9 expression,the positive expression rate of VEGF was 52.54%(31/59).The difference was statistically significant(P<0.05).There was a positive relationship between the expression of HOXB9 and VEGF in colorectal carcinoma tissues.The VEGF immunohistochemical score was increased with the increase of HOXB9 score,that is the express level of VEGF was increased with the increase of HOXB9 expression.4.In colorectal carcinoma tissues,HOXB9 and microvessel density had different expression levels,the expression of HOXB9 was increased,and the CD34-MVD value was also increased.In colorectal carcinoma tissues with negative HOXB9 expression,the CD34-MVD value is(12.786±2.307),when the expression of HOXB9 was positive,the CD34-MVD value was(18.012±1.798).The MVD values of colorectal carcinoma tissues with different HOXB9 expression levels were significantly different(P<0.05).There was a positive relationship between the expression of HOXB9 and the CD34-MVD value in colorectal carcinoma tissues.The CD34-MVD value increased with the increase of HOXB9 immunohistochemical score.Conclusion: The expression of HOXB9 was upregulated in colorectal carcinoma tissues,and its expression was closely related to lymph node metastasis,the depth of invasion and TNM stage of the colorectal carcinoma tissues.The correlation of HOXB9,and VEGF and micro-angiogenesis in colorectal carcinoma tissues was positively.The high expression of HOXB9 can promote micro-angiogenesis in colorectal carcinoma tissues.HOXB9 is expected to be a biomarker for the screening and therapeutic effect of bevacizumab,an anti-angiogenic target drug.The clinical detection of HOXB9 expression in colorectal cancer patients is helpful to the selection of anti-angiogenic drugs,such as bevacizumab.