Experimental Research On The Expression Of CD151 Gene In Combination Of Targeted Therapy Of Colorectal Cancer With Bevacizumab

Objective: To explore the CD151 protein expression in case of influence on microvascular growth,as well as the judgment of CD151 protein expression and anti VEGF targeted drugs if there is a synergy,after colon cancer was treated with conventional chemotherapy and anti VEGF targeted drugs.Methods: Detect each group of CD151 protein expression respectively in transplanted tumor tissue by Western blot method.Using CD34 markers and the method of immunohistochemical vascular endothelial cells to observe the transplanted tumor tissue of the microvascular density(MVD)under optical microscope.Results:1 Under the mirror HT29 colon cancer cells were spindle type or irregular triangle.They have different size and shape.Their nuclear are enlargement and abnormal,the nucleus and cytoplasm ratio is close to 1:1.All the cells gathered in one clangorous flocked to the scoop.These adherent cells extend smoothly,and they can be used for the follow-up study.2 After inoculating HT29 colon cancer cells,all nude mice tumor’ survival status are tolerated.In the 7th day,groups of nude mice can be observed larger tumors which is visible to the naked eye.Groups of nude mice from the 8th day were given medicine filling and feeding.Group A happened two Death,the death rate is 13.33%.Group B happened one death,the death rate is 6.67%.Group C happened one death,the death rate is 6.67%.3 Groups of transplanted tumors showed a significant difference on the product growth curve after six days’ transplantation.The control group’ transplanted tumor growth rate is significantly higher than chemotherapy group and combination group.Comparing volume of the 21 th day’s tumors,volume of transplanted tumors of group A is 1187±219.69 mm3,Group B’ volume is 757.2±69.68mm3,Group C’ volume is 532.3±40.12mm3,statistical results is P<0.05.Groups of transplanted tumor tumor weight contrast: Group A tumor transplanted tumor weigh 1.42±0.26 g,Group B tumor transplanted tumor weigh 0.901±0.082 g,Group C tumor transplanted tumor weigh 0.629±0.04 g,Statistical results is P < 0.05.The chemotherapy group and combination group’ tumor weight significantly higher than the control group transplanted tumor,and the transplantation tumor chemotherapy group focuses on the combination therapy group.4 Using the Western blot method to detect each group’ CD151 protein expression,the results show that CD151 protein expression in tumor tissue of group A of nude mouse’ transplantation compared to group B and group C,CD151 protein expression in transplanted tumor tissue decreased obviously.the group B’s CD151 protein expression in transplanted tumor tissue was higher than group C’s CD151 protein expression in transplanted tumor tissue volume,statistically significant(P<0.05).Group A protein expression of fluorescence intensity value is(1.13±0.082);Group B protein expression of fluorescence intensity value is(0.92±0.054);Group C protein expression of fluorescence intensity value is(0.75±0.031).5 Groups of transplanted tumor tissue’s microvascular count(MVD)after CD34 markers vascular endothelial cells: group A(70.04±9.80)and group B(59.95±6.54),group C(43.46±1.98).In group B and group C,compared them with group A,microvascular density(MVD)decreased obviously,and group C microvascular count was associated with significantly lower than that of group B microvascular count.Conclusions:1 In the chemotherapy of colorectal cancer tissue,the expression of CD151 protein is on the decline,accompanied by the decrease of tumor angiogenesis.Prompting CD151 protein may be involved in regulation of tumor angiogenesis.2 Receiving the combination of targeted drugs vascular proliferation in colorectal cancer tissue density was significantly lower than the pure chemotherapy group.And we considered CD151 protein and anti-tumor angiogenesis targeted drugs have synergy in terms of tumor microvascular generation.3 Further clarifying the angiogenesis in specific regulatory mechanism of colorectal cancer tissue can be better inhibiting colon cancer tumor growth and metastasis by giving appropriate intervention measures.