Study On The Relationship Between S100A9 Protein Expression And Proliferation And Metastasis Of Cholangiocarcinoma Cell

Objective Our previous experiments have revealed that the expression of S100A9 in cholangiocarcinoma tissue is higher than that in paracancerous tissues.Its abnormal expression in cholangiocarcinoma suggests that it may be involved in the occurrence and development of cholangioc arcinoma.To further clarify the mechanism of cholangiocarcinoma invasion and metastasis,the effects of down regulation and deletion of the S100A9 gene on the proliferation and metastasis of cholangiocarcinoma cell line QBC939 are investigated in this study.Methods Cholangiocarcinoma cell line QBC939 was cultured routinely.Then,the expression of the S100A9 gene in cholangiocarcinoma cells was detected using the qPCR real-time quantitative PCR detecting system.QBC939 cells were transfected with RNA interference target sequence containing S100A9 gene and no-load control lentivirus,respectively.The expression of green fluorescent proteins and the transfection rate of viruses were observed under an inverted microscope.The expression of S100A9 gene in cholangiocarcinoma QBC939 cells was detected using real-time fluorescent nucleic acid amplification assay.The proliferation of QBC939 cells by MTT assay,and the invasion and metastasis of QBC939 cells by Transwell,invasion and scratch assay.Results⒈Compared with normal biliary cells,the S100A9 gene was highly expressed in cholangiocarcinoma cell line QBC939.⒉ After cholangiocarcinoma QBC939 cells were transfected with S100A9-shRNA lentivirus,the mRNA expression of S100A9 gene was inhibited,and knockdown efficiency reached 90.9%.⒊Transwell,invasion and scratch preassay showed that QBC939 cells with high S100A9 expression had weak invasion and metastasis ability.⒋ MTT results showed that compared with the control group,the growth rate of QBC939 cells transfected with lentiviral vector S100A9-shRNA did not decrease significantly.Conclusions⒈ The expression of S100A9 in cholangiocarcinoma cell line QBC939 increases,it suggests that S100A9 has a certain relationship with the occurrence of cholangiocarcinoma.The invasion and metastasis ability of QBC939 cells with high S100A9 expression is weak.⒉Interference with the S100A9 gene cannot limit the proliferation of cholangiocarcinoma cell line QBC939.