Construction And Performance Of Intelligent Antibacterial Surface

Health care-associated infections are the world’s major public health problems and lead to serious consequences,such as increased economic burden,increased patient morbidity and mortality.At present,the main method for solving such problems in clinical is antibiotic therapy.Although the use of antibiotics has greatly reduced the morbidity and mortality caused by bacterial infections,the emergence of bacterial resistance has seriously threatened the lives of patients.The study of new antibacterial agents provides new ideas for solving antibiotic resistance.However,the direct use of new antibacterial agents such as quaternary ammonium salts,antibacterial peptides will produce serious biological toxicity.Therefore,constructing an intelligent response antibacterial strategy on the surface of the material,on the one hand,can realize the corresponding function switching according to the changes of the surrounding environment,on the other hand,it can maintain the good biocompatibility of the material.This paper aims at the increasingly serious problem of bacterial infection of materials,and develops anti-bacterial infection surface.Although the existing antibacterial surface has excellent antibacterial properties,there are still anti-adhesion and sterilization functions that interfere with each other.The intelligent release surface focuses on the release sterilization process,which cannot solve the problem of surface biocompatibility under non-infected conditions.Therefore,this paper builds an intelligent responsive antibacterial system,which realizes the on-demand activation of antibacterial and biocompatibility,which provides new ideas for expanding the research of responsive antibacterial surfaces.Part I: Construction and properties of bacteria-activated antibacterial surfaces.In this study,the surface was constructed by UV grafting,the bee venom peptide was fixed to the surface,and then the charge conversion mechanism was introduced into the design of the antibacterial surface.The detection surface was successfully constructed using Fourier infrared spectroscopy and water contact angle.Bacterial experiments proved that the killing rate against Staphylococcus aureus(S.aureus)was about 94.16 %,and the killing rate of Escherichia coli(E.coli)was about 97.46 %.The biocompatibility experiment proved that the hemolysis rate of the surface exposed bee venom peptide was about 8.69 %,and the surface hemolysis rate after modification by charge reversal molecules was about 0.56 %;in vivo tissue compatibility studies did not find any Verification of infection,and antibacterial properties in vivo also reached more than 90%.Part II: The construction and performance of p H response adaptive antimicrobial surfaces.In this study,photoinitiated graft polymerization was used to fix the quaternary ammonium compound and the p H-sensitive monomer 2,3-dimethylmaleic anhydride on the surface,and a p H-responsive adaptive antibacterial surface was successfully construct.Using fourier infrared spectroscopy,water contact angle and so on detect the surface.Experiments show that the sterilization rate against S.aureus is about 99.32 %,the anti-adhesion rate reaches 90 %,the surface hemolysis rate is 0.26 %,almost does not destroy the blood cell morphology,and shows excellent tissue and antibacterial properties in the body performance.