Effects Of Different Antibiotic Regimens On Body Mass,Lipid Metabolism,Insulin And Gut Microbiota In Mice Early Life

Objective: To investigate the effects of antibiotics on body mass,lipid metabolism,insulin,and gut microbiota in mice in early life.Methods: 144 2-week-old Kunming mice were randomly divided into six groups: intravenous administration control group(saline injection),intravenous administration of single antibiotic(cefotaxime),intravenous administration of antibiotics(cefotaxime combined with ampicillin sulbactam),oral control group(saline),oral administration of single antibiotic(cefotaxime)and oral administration of antibiotics.In the drug group(cefotaxime combined with ampicillin sulbactam),24 rats in each group were given corresponding intervention for one week,and then fed for three months.Eight mice in each group were killed at the end of the first month,the second month and the third month respectively.The gut microbiota body weight,lipid metabolism,lipopolysaccharide and insulin of each group were analyzed.Compared with CIG and CAIG,NIG had significantly more bacteroidetes than CIG and CAIG,and the p value was 0.030,indicating a statistically significant difference.Compared with CAIG,CIG has a relatively large proportion of cocci,such as Deinococcus-Thermus,with a p value of 0.031.Compared with CPG and CAPG,the number of Fusobacteria in NPG was relatively small,with a p value of 0.000,indicating a statistically significant difference.There was no significant difference in the composition of CPG and CAPG.Results: Compared with the control group,the body mass of the mice in the intravenous group and the intravenous group increased significantly at the end of the first month and the second month(p < 0.05).The insulin level of the mice decreased first and then increased(p < 0.05).The lipid metabolism showed that HDL decreased first and then increased(p < 0.05).LDL,TG and LDL increased first and then decreased(p < 0.05).There was no significant difference in TC among the three groups(p > 0.05).There were no significant differences in body weight,insulin level and lipid metabolism between the two groups.(p>0.05)Conclusion: Intravenous application of antibiotics in early life can increase body mass and epididymal fat in mice for a long time,but the changes of liver and perirenal fat quality are not significant.It also increase LDL and TG levels,decrease HDL levels and decrease insulin levels in mice over a long period of time.This effect was not obvious in peroral administration.The final intestinal flora composition of mice after antibiotic intervention in early life was decreased by bacteroidetes,This change was more obvious in the intravenous group,but not in the peroral group.