Study On The Role Of Carbon Point In Non-Alcoholic Fatty Liver Disease Related To Iron Overload

In recent years,with the rapid development of technology and economy,people’s living habits have undergone great changes,and diseases related to lipid metabolism have become an important issue that plagues human health.Non-alcoholic fatty liver disease(NAFLD)is a representative metabolic lifestyle disease with high incidence and many complications,and the declining population is gradually becoming younger,which has become a major disease that endangers human health and social development.Recent studies have found that iron overload in the body is closely related to the incidence of NAFLD.The large accumulation of iron in the body can not only cause liver fat degeneration,but also increase oxidative stress,aggravate inflammol/Lation and worsen lipid metabolism.Nano-carbon dots(CDs),as a new type of fluorescent carbon nano-materials,have good biological properties and high biocompatibility and are widely used in the field of biology.The latest research report found that CDs have a strong chelating ability for iron ions.Therefore,we introduced iron ion-chelating CDs into the treatment of iron overload NAFLD.In this study,through the treatment of juvenile zebrafish with high-speed rail and thioacetamide,an animal model of NAFLD disease was established,and iron chelated CDs were given for intervention to explore the role of CDs in NAFLD disease and its regulatory mechanism.The results found that: High-speed rail combined with thioacetamide treatment of juvenile zebrafish can successfully induce NAFLD model.The model group showed: liver lipid deposition,larger size,increased hepatocyte gap,nucleus shift,fatty large vacuoles,triglyceride and total cholesterol levels were significantly higher than the normal control group.After the intervention of CDs,the lipid deposition in the liver of NAFLD model decreased,and basically returned to normal levels.The cell morphology was also similar to that of the control group,the liver cell gap was reduced,the fatty large vacuoles disappeared,and the liver volume returned to normal;the triglyceride and total cholesterol levels dropped to normal levels.Further molecular biology research found that CDs intervention can reduce fatty acid synthesis,promote the oxidative decomposition of fatty acids,and thereby improve lipid deposition in the liver.At the same time,CDs intervention can relieve cellular oxidative stress and endoplasmic reticulum stress response,induce the production of anti-inflammol/Latory factors and inhibit the expression of pro-inflammol/Latory factors,thereby improving the occurrence and development of fatty liver.This research provides a theoretical basis for the prevention and treatment of NAFLD related to iron overload,and provides a new idea for the biomedical application of nanomaterials.