| Objective1. To study whether there is being iron overload in the patients with liver diseases.2. To assess the predict value of the serum level of iron and ferritin for iron overload.3. To investigate the relationship between iorn and indicators of hepatic fibrosis.4. To observe the level of blood lipid and lipoprotein in the patients with liver cancer or benign liver diseases.Methods1. The levels of hyaIuronicacid(HA),Iaminin(LN),procollagen type III (hpc-III),collagen type IV(IV- C) were determined by radioimmunoassay.2. To examine the serum levels of iron, ferritin by atomic absorption spectrum and radioimmunoassay respectively.3. The liver tissue stained by haematoxylin-eosin(HE) and Perl's Prussian.4. To analyze the relationship between serum iron, ferritin withliver iron overload in patients with benign ( fatty liver ,liver cirrhosis, polycyst liver etc.) or malignant liver diseases. To observe the serum biochemistry index of the patients and the normal healthy group,Results1. Among the patients with benign liver diseases, the levels of serum iron and ferritin in the patients with AFL (29.67± 13.52umol/L;386.20±156.42ng/ml)are obviously higher than that of normal group (19.41±11.74umoI/L; 280.18± 229.35mg/ml)( P<0.05 );but there is no statistic difference in patients with liver cirrhosis (P>0.05) .The level of serum ferritin (598.03±312.26 ng/ml)in patients with HCC is obviously higher than that of normal group (P<0.01),but there has no statistic difference in the level of serum iron between the patients with normal individuals (P>0.05).2. Hyaluronicacid(HA),laminin(LN),procollagentype(hpc III),collagentypeIV(IV-C) concentrations in the patients with liver cirrhosis ,AFL, and hepatic cellular cancer(Gl)are obviously higher than those in the control (P<0.05). Compare the levels of the indicators of liver fibrosis between the hepatic cellular cancer(G2) with control group, there are no statistic differences (P>0.05).3. Stained by Perl's Prussian: iron in tumor tissue is significantly lower than that in benign liver tissue. There isno iron granule found in hepatic cancer tissue of the 25 patients. However, there are being iron granules in the liver tissue of six patients (4 cases of fatty liver and 2 of liver cirrhosis) among sixteen patients with benign liver diseases, the positive rate is 37.5%, and the levels of the serum iron and ferritin are higher than the normal values.4. Compared to the control group(10.5±5.7umol/L,143.3± 71.9ng/ml),the serum levels of iron and ferritin are obviously higher in patients with AFL(20.9±9.3 umol/L ,217.6±71.8 ng/ml) (P<0.01),but there are no statistic differences in the patients with NAFL (11.5± 6.15umoI/L;149.3±72.1 ng/ml ) , there are statistic differences between NAFL and AFL.5. There are no correlations between iron ,ferritin with HA,hpc-III,IV-C in the patients with benign liver diseases and hepatic cellular cancer, but serum iron and serum ferritin have the correlations to the serum LN. LN(Y)= 160.3+3.09×X(serumiron),r=0.439,P=0.03;LN(Y)=149.95+0. 160×X(serum ferritin), r=0.476,P=0.016.6. The levels of serum TC,TG,HDL-C,LDL-C in patients with liver cirrhosis and liver cancer are obviously lower than those in control group (P<0.05) .To compare the HCCG1 group with the HCCG2 group, there are statistic differences too.(P<0.05)Conclusions1 .The patients with AFL often have high levels of serum iron and ferritin.To compare with the normal group, there has statisticdifference(P<0.05). 2. Compared with the control group the level of serum ferritin isobviously higher in the patients with HCC. But the level of serum ironis not. 3.Hyaluronicacid(HA),Iaminin (LN),procollagen typeIII(hpc-III),collagentypeIV(IV-C) concentrations in the patients with benign liver diseasesor HCC(Gl)are obviou... |
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