Effects Of Rab5 On The Proliferation And Differentiation Of Intestinal Stem Cells In Drosophila Melanogaster

The gastrointestinal tract is not only the body’s largest digestive organ,but also the body’s largest immune organ and detoxification organ,and its health status directly affects life span.Drosophila is widely used in the research of developmental biology and genetics because of its convenient operation,small size,easy breeding,short reproductive cycle,few chromosomes,and many mutants.the morphological structure of Drosophila midgut and mammalian small intestine are very similar,and its genes in the signal pathway are relatively conserved,making Drosophila an important model organism for intestinal research.Rab5 is mainly localized in early endosomes,plays a key role in the regulation of vesicle transport in early endocytosis,and participates in phagocytosis,autophagy,cancer cell migration and cell proliferation.Through the hybridization of Rab5 RNAi Drosophila and Gal4 Drosophila which specifically expressed in stem cells and differentiated cells and multiple antibodies were used for immunostaining to analyze the number of cells,cell size and expression levels of related signaling pathway genes.These methods were used to explore the effect of Rab5 protein on the proliferation and differentiation of Drosophila intestinal stem cells.The results showed:1.Rab5 deletion leads to a large amount of proliferation of precursor cells and inhibits the differentiation of precursor cells into intestinal enteroendocrine cells.2.Rab5 deletion leads to disordered intestinal epithelial cell arrangement,shortened copper cell area,thickened middle hindgut,and destroyed the integrity of intestinal epithelial cells.3.Rab5 deletion affects the expression of genes in EGFR,JNK and Notch signaling pathways.This study explored the effects of Rab5 protein on the proliferation and differentiation of Drosophila intestinal stem cells,and provided a theoretical basis for diseases related to intestinal metabolism.