Effects Of Metformin On Intestinal Flora And Metabolism In Mice

Objective:To explore the correlation between the hypoglycemic effect of metformin and intestinal microflora in mice,as well as the changes of physiological activity in mice after administration,in order to guide clinical medicationMethods:The experiment selected 96 C57BL/6J mice of 8 weeks of age,divided into normal mouse control group(N),normal mouse metformin treatment group(NM),diabetes model control group(D)and diabetes model metformin treatment group(DM).Among them,N group and NM group mice were fed with normal diet,D group and DM group mice were fed with high fat diet.High-fat diet mice were first made a pathological model of type 2 diabetes,confirmed as a model and then administered metformin.In this experiment,42 mice were moulded,and the mold formation rate was 91.3%.The mice in NM group and DM group were given 300 mg/kg concentration of metformin hydrochloride solution 0.2 ml/time/day,and the mice in N group and D group were given the same amount of normal saline 0.2 ml/time/day,and all mice were given the same amount of gavage once per day Six weeks.The weight,blood sugar,diet and water consumption of mice were recorded regularly every week.the pcr-dgge method was used to analyze and compare the mouse fecal samples for each week during the experiment.At the 3rd,4th,5th,6th week of the experiment,the serum of each 6 mice in 4 groups of mice was collected,and the serum samples were tested for vitamin K content.At the sixth week of the experiment,the liver and jejunum of the diabetic mice were stained with HE and then the morphological changes were observedResults:1 During the 6 weeks of the experiment,there was no significant difference in body weight between normal mice and diabetic model mice after taking metformin and the control group(P>0.05)2 To a certain extent,metformin can inhibit the appetite of mice and affect the amount of food and water consumption of mice3 The use of metformin did not significantly change the diversity of intestinal flora in normal mice with normal diet,and the effect of metformin on intestinal flora in mice was not obvious4 After taking metformin for 4 weeks,the intestinal microflora of the diabetic model mice fed with high-fat diet changed significantly.Compared with the(D)mice in the diabetic model control group,the drug changed the diversity of intestinal microflora in the treatment group(DM)mice,and there was a significant difference in Shannon index between the two groups This is still the case in week 5 and week 65 The content of vitamin K in serum of normal mice and diabetic model mice increased to a certain extent after administration of metformin,and at the 6th week of the experiment,there was significant difference in serum vitamin K between normal mice control group and normal metformin treatment group(P<0.01).There was also a significant difference in the value of serum vitamin K between diabetic model control group(D)and diabetic model metformin treated(DM)mice(P<0.05)6 After metformin treatment of diabetic model mice for 6 weeks,it was found that compared with the diabetic model control group,the liver of(DM)mice in the treatment group had fewer fat vacuoles,less lipid deposition and neat arrangement of cells,and less steatosisConclusion:1 Metformin can reduce the food intake and water intake of normal and diabetic model mice to a certain extent2 Metformin has no effect on the intestinal flora of normal mice3 Metformin can significantly change the intestinal flora of diabetic model mice in the fourth week4 Metformin can increase the content of vitamin K in serum of normal mice and diabetic model mice5 Metformin can improve hepatic steatosis in diabetic mice.