| OBJECTIVE:To study the protective effect of exercise pretreatment on ischemia-reperfusion injury in MCAO rats,and explore the mechanism of neuro-protection,and provide a new idea for clinical treatment of cerebral ischemia-reperfusion injury.METHODs:Ninety adult male Sprague-Dawley rats(body weight 240±20g)were randomly divided into three groups.That is Sham operation group,MCAO model group and exercise pretreatment group,with 30 rats in each group.The exercise pretreatment group was practiced for 4 weeks before modeling.The model group and the exercise pretreatment group were treated with thread embolism of middle cerebral artery.Rats were embolized for 2 hours,and the plug was removed for blood flow reperfusion.Neurological function was evaluated to detect the degree of neurological damage.Immunofluorescence staining was used to observe the loss of environment in cortical ischemic penumbra neurons.Western blot was used to detect ischemic hemisphere TLR4 and NF-kB protein expression.RESULTS:Longa score showed that the score of MCAO group was significantly higher than that of sham group(P<0.001).Compared with the MCAO group,the Moter+MCAO group scores significantly decreased(P<0.05).The results were statistically significant.The Berderson score showed a significant increase in the MCAO group compared with the Sham group(P<0.001).Compared with the MCAO group,the Moter+MCAO group scores significantly decreased.(P < 0.001),the results were statistically significant.The results of the climbing rope test showed that the MCAO group score was significantly higher than the Sham group(P<0.001);compared with the MCAO group,the Moter+MCAO group.The score was significantly lower(P < 0.001)and the results were statistically significant.Similar results were obtained for screen training,accelerated rod experiments,and balance beam experiments.Immunofluorescence and toluidine blue were used to detect the number of neurons.Exercise preconditioning significantly improved MCAO-induced rat cortical neuron loss compared with Sham group,MCAO group neuron number decreased significantly(P<0.001);compared with MCAO group The number of neurons in the Moter+MCAO group was significantly up-regulated(P<0.01),and the results were statistically significant.Exercise pretreatment significantly inhibited the release of inflammatory factors from rat cortical tissue induced by MCAO.Exercise pretreatment cansignificantly improve the neurological score,reduce the loss of nerve cells,and improve the neurological damage.Real time PCR was used to detect the expression of inflammatory factors.Compared with the Sham group,the MCAO group showed a significant increase in expression;compared with the MCAO group,the expression of the Moter+MCAO group was significantly down-regulated,and the results were statistically significant.The expression levels of TLR4 and NF-κB protein were up-regulated in MCAO-modeled animals;the expression levels of two proteins in exercise-pretreated group were significantly lower than those in MCAO group.This result suggests that exercise preconditioning may reduce the damage of brain tissue by down-regulating the expression of TLR4 and inhibiting the transcriptional regulation of NF-κB to improve the inflammatory response of the lesion.CONCLUSIONs:1.Exercise pretreatment can significantly improve the neurological damage of rats induced by MCAO.2.Exercise pretreatment can effectively inhibit the release of inflammatory factors induced by MCAO through the TLR4/NF-κB signal transduction pathway. |
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