Effect Of Valerian Ligusticum Pill On Angiogenesis And Neurogenesis After Injury Of Cerebral Ischemia Reperfusion In Rats

Part I The protective effect of Valerian Ligusticum Pill?VLP?on cerebral ischemia reperfusion injury in rats.Objective:To explore whether VLP has a protective effect on acute cerebral ischemia reperfusion injury.Methods:Rats were setted up into sham operation group,model group,VLP-low group,VLP-high group and Nimodipine group,established models of MCAO,reperfusion after 30 minutes'ischemia.When all rats were awake,gave them first neurological function score,afterward gave medicine or distilled water by different groups.Seven days later,all rats were given second neurological function score,calculated the improvement of neurological function.After that,rats were sacrificed to test the cerebral infarction volume percentage.Results:In comparison with model group,improvement of neurological function in treatment groups was increased?P<0.05,P<0.001?,and the cerebral infarction volume percentage was significantly reduced?P<0.001?.Conclusion:VLP helped improve the neurological function,reduce the cerebral infarct volume percentage of rats after cerebral ischemia reperfusion injury,and showed a certain protective effect on the brain.Part II Effect of VLP on angiogenesis after injury of cerebral ischemia reperfusion in rats.Objective:To investigate whether the VLP'protective effect on cerebral ischemia reperfusion injury has an association with angiogenesis.Methods:Rats were treated with the same grouping,modeling,scoring and administration as Part I.Seven days later,testing the expression of protein VEGFR2 in rats'cerebral cortex infarction around by Western blot,and the quantity of neovascularization by immunofluorescence double staining method.Results:In comparison with model group,the expression of VEGFR2 in VLP-low group rose?P<0.05?,VLP-high and Nimodipine group rose significantly?P<001?;in comparison with model group,the quantity of neovascularization in VLP-low and Nimodipine group increased?P<0.05?;VLP-high group increased significantly?P<0.001?.Conclusion:VLP could play a neuroprotective role by promoting VEGFR2 expression and the formation of new blood vessels in cerebral cortex infarction around,while VLP-high(50mg�kg^-1)group showed more obvious.Part?Effect of VLP on neurogenesis after injury of cerebral ischemia reperfusion in rats.Objective:To investigate whether the VLP'protective effect on cerebral ischemia reperfusion injury has an association with neurogenesis.Methods:Rats were setted up into sham operation group,model group and VLP-high group,treated with the same modeling,scoring and administration as Part I.Seven days later,testing the expression of Nestin,DCX,NeuN in rats'cerebral ischemic penumbra by immunofluorescence single staining method.Results:In comparison with model group,expression of Nestin in cerebral ischemic penumbra in VLP-high(50mg�kg^-1)group rats rose significantly?P<0.001?,so did the expression of DCX and NeuN.Conclusion:V LP could promote the expression of Nestin,DCX and NeuN in rats'cerebral ischemia penumbra,stimulate the endogenous neurogenesis,and thus play a neuroprotective effect.