Expression Of MNK1 In Non-Small Cell Lung Cancer And Its Relationship With Clinicopathological Features And Prognosis

Objective:By analyzing the expression of MNK1 in non-small cell lung cancer and normal tissues and paracancerous tissues,and its relationship with clinicopathological features and survival time,to explore the role of MNK1 in the occurrence and development of non-small cell lung cancer and to provide new ideas for early diagnosis and prognosis of non-small cell lung cancer.Methods: Western blotting detection of MNK1 expression in five lung cancer cell lines and one normal bronchial epithelial cell line;97 pairs of non-small cell lung cancer(NSCLC)and paracancerous tissues were collected from the Fourth Affiliated Hospital of China Medical University between December 2007 and June 2015.The expression of MAK1 protein was detected by immunohistochemistry.The statistical data were analyzed by SPSS17.0 software,with P < 0.05 as the significance level.T-test was used to analyze the expression differences of MNK1 in non-small cell lung cancer cells and normal cells.The chi-square test was used to analyze the relationship between MNK1 expression and various clinicopathological features of non-small cell lung cancer.The Kaplan-Meier method was used to calculate the survival rate.The survival curve was compared with the Log-rank test.COX proportional hazards regression analysis of MNK1 expression and clinicopathologic features may influence prognostic factors in patients with non-small cell lung cancer.Result: 1.Compared with normal bronchial epithelial cells(16HBE),MNK1 expression levels in five NSCLC cell lines(H1299,SK-MES-1,LTE,LK2,A549)were significantly higher,and the difference was statistically significant(P=0.007,0.0087,0.0003,0.0187,0.0024).2.The expression of MNK1 protein was mainly in the cytoplasm of cells.The positive rates of MNK1 protein expression in non-small cell lung cancer tissues and adjacent tissues was 51.5%(50/97)and 22.7%(22/97),respectively.The difference was statistically significant(P =0.010);3.The 3-year survival rate of 97 non-small cell lung cancer patients was 80.3% and the 5-year survival rate was 68.4%.The 3-year survival rate of patients with high expression of MNK1(n=50)was 68.0%,the 5-year survival rate was 56.3%,and the 3-year survival rate f patients with low expression of MNK1(n=47)was 93.5%,and the 5-year survival rate was 81.2%.There were statistically significant differences between the two groups(P =0.003);4.The chi-square test was used to analyze the relationship between the expression of MNK1 and clinicopathological features.Results: The expression level of MNK1 was related to lymph node metastasis(P=0.048)and clinical stage(P=0.041),but was not related to age,sex,smoking,T stage,pathological type,and differentiation(P > 0.05);5.Univariate Kaplan-Meier method analysis of 97 cases of non-small cell lung cancer showed that the expression level of MNK1(P =0.003),differentiationdegree(P = 0.005),postoperative chemotherapy(P=0.043)were associated with the prognosis of non-small cell lung cancer,While age,gender,smoking status,lymph node metastasis,T stage,clinical stage and pathological type were not correlated with the prognosis of non-small cell lung cancer;Further COX multiple regression analysis showed that the expression level of MNK1(P =0.018),differentiation degree(P =0.001),postoperative chemotherapy(P =0.004)were one of the prognostic indicators that affect the prognosis of non-small cell lung cancer.Conclusion:1.MNK1 is highly expressed in non-small cell lung cancer and cancer tissues.2.The expression level of MNK1 was significantly correlated with lymph node metastasis and clinical stage in patients with non-small cell lung cancer.3.The level of MNK1 expression,clinical stage,differentiation,and postoperative chemotherapy are one ofthe indexes that affect the prognosis of NSCLC.