Correlative Study Among Gene Polymorphisms,Factor Ⅷ Activity And VWF Ag In High-incidence Thrombotic Diseases

The threat of thrombotic diseases to human health is increasingly severe,searching for the methods to prevent and treat the diseases is demanded urgently.Recently foreign researches suggest that von Willebrand factor(VWF)associates with thrombosis closely,plasma factorⅧ(FⅧ)has been recognized as the independent risk factor of thrombosis and the reports illustrate that SNPs relate to FⅧ activity and VWF antigen in Caucasian and African races.Furthermore,phenomenon can affect the progression of thrombotic diseases.Based on the above reasons,our subject is required to discuss the following items: the influence of environments,lifestyles and genetic factors to thrombotic diseases.Besides that,we need to figure out whether Single Nucleotide Polymorphisms(SNPs)relate to FⅧ activity and VWF antigen.Methods: Collection of clinical blood samples,separation and preservation of blood cells and plasma,sorted by CVD group,tumor group and control group.Distraction of genome DNA by adsorption column.Genotyping of 20 SNPs from 9 genes including factor Ⅷ、VWF、ABO、SCARA5、STAB2、STX2、CLEC4M、STXBP5、KCNE2 by imLDR technology.Testing of plasma FⅧ activity by one-period method.Detection of VWF antigen levels by ELSA.Results:1.There are statistical differences of FⅧ activity among CVD group,tumor group and control group(p<0.05).Highest in tumor group,lowest in CVD group.And there are statistical differences of VWF Ag among CVD group,tumor group and control group(p<0.05).Highest in CVD group,lowest in control group.2.In CVD group,FⅧ activity is relevant to platelet counts statistically(p<0.05)in positive correlation(r=0.285).VWF Ag is relevant to coronary diseases and their family history statistically(p<0.05).The patients which suffering from family history have higher VWF Ag levels.rs11780263 of SCARA5 is relevant to FⅧ activity(p=0.030).3.In control group,there was statistical differences between FⅧ activity and sex.The FⅧ activity of female is higher than male.FⅧ activity is relevant to age(p<0.05)by relevance analysis in positive correlation(r=0.26).rs11780263 of SCARA5 and rs4981022 of STAB2 is relevant to VWF Ag levels(p<0.05,p<0.01).In arrhythmia group,rs4981022 of STAB2 is relevant to VWF Ag and FⅧ-VWF ratio(p<0.05,p﹤0.05).rs868875 of CLRC4 M is relevant to FⅧ activity(p﹤0.05).rs9390459 of STXBP5 is relevant to FⅧ-VWF ratio(p﹤0.05).4.In tumor group,rs11780263 of SCARA5 is relevant to FⅧ-VWF ratio(p<0.05),STX2rs7978987 is relevant to VWF Ag levels(p<0.05).The frequency of rs2726953 A in SCARA5 is significant different between tumor group and control group(p<0.05).5.SNPrs1222929 of STAB2 in male is relevant to FⅧ activity(p<0.05).Conclusion:1.Plasma FⅧ activity and VWF Ag levels in CVD group,tumor group and control group vary in some extent.That results suggest that the two indexes participate in pathogenesis of thrombotic diseases.2.We believe that many SNPs including FⅧ,VWF and other genes relate to Plasma FⅧ activity and VWF Ag levels.It illustrates that plasma FⅧ activity and VWF Ag levels of high-risk thrombotic diseases may have complicates inner genetic mechanisms in the population of northwestern region.That finding shall provide the theoretical basis for prevention and therapy of thrombosis.3.The effects of Tobacco,alcohol and environment to plasma FⅧ activity and VWF Ag levels are not notable.