Effect Of Asymmetric Tension On Biomechanics And Metabolism Of Vertebral Epiphyseal Plate In A Rodent Model Of Scoliosis

Objective: 1.The new design rodent model of scoliosis was performed by Sprague-Dawley rats;2.To analysis differences on the convex and concave sides,finite element analysis was carried out to determine the mechanical stress;3.Protein expression on epiphyseal cartilage was evaluated by western blot;4.Micro-CT was taken to evaluate the bone quality of vertebral on both sides;5.By analyzing scoliosis asymmetric tension to understand their role and pathogenic mechanisms.METHODS: 1.8 SD female rats were chosen to found model of scoliosis;2.Scoliosis digital computer simulation was used to study the asymmetric distribution of tension;3.Expression of type II collagen,type X collagen,Sox9,RUNX2,VEGF and aggrecan on concave and convex sides was measured;4.Trabecular micro-architecture was measured.RESULTS: 1.All rats underwent X-ray radiography and had successful scoliosis curve;2.Finite element analysis of stress applied to the spine and the epiphyseal plate was asymmetric,convex side has a greater stress;3.The protein expression was expressed significantly more on the convex side;4.The asymmetrical development of trabecular bone was significantly different and the concave side had significantly lower value in the BV/TV and Tb.N,but higher value in the Tb.Sp.CONCLUSIONS: These results suggested that there was an asymmetrical vicious circle in IS.Asymmetric tension contributed to changes in protein expression and bone quality,ultimately resulting in asymmetry of anatomy.It might give some new insight into the pathogenic mechanism of IS.