Analysis Of Changes In Intestinal Dominant Flora In Patients With Functional Abdominal Pain Syndrome

Objective:In this study,based on the intestinal flora,real-time quantitative PCR was used to quantitatively analyze Bifidobacteria,Lactobacillus,Escherichia coli and Enterococcus in the intestinal tract of patients with functional abdominal pain syndrome(FAPS).Patients with abdominal pain syndrome(FAPS)have dysbacteriosis,which provides new clues to the pathogenesis of functional abdominal pain syndrome(FAPS)and seeks new targets for the treatment of functional abdominal pain syndrome(FAPS).Methods : Prospective,randomized,controlled studies were used to divide the subjects into case groups and healthy controls.The case group was treated with 40 patients with functional abdominal pain syndrome(FAPS)in the digestive department of our hospital.The healthy control group was 40 healthy subjects with no chronic digestive tract disease at the same time.The fresh feces of the case group and the healthy control group were collected,and the fecal genomic DNA was extracted for real-time fluorescent quantitative PCR reaction.The reaction was carried out by using the extracted genomic DNA as a template and using the 16 S rRNA gene sequences of the four target bacteria to be tested as specific primers.After the reaction,the copy number of the two groups of samples to be tested was calculated by PCR software,and the copy number was used as the quantitative result.The results were statistically analyzed by SPSS 20.0 statistical software.The differences between groups were compared by independent sample t test.P< 0.05 was considered statistically significant.Results:Compared with the healthy control group,the number of bifidobacteria(5.66±0.54 vs 5.87±0.58)and lactobacilli(6.96±0.44 vs 7.10±0.38)in the feces of patients with functional abdominal pain syndrome(FAPS)was significantly reduced,P<0.05.The difference was statistically significant.There was no significant change in the number of E.coli(9.04±0.48 vs 9.02±0.42)and Enterococcus(4.79±0.54 vs 4.80±0.62),P>0.05.The difference was not statistically significant.Conclusions:The number of bifidobacteria and lactobacilli in feces of patients with functional abdominal pain syndrome(FAPS)was significantly lower than that of healthy people,indicating a change in the dominant intestinal flora of patients with functionalabdominal pain syndrome(FAPS).Probiotic preparations such as bifidobacteria and lactobacilli may become drug targets for the treatment of functional abdominal pain syndrome(FAPS).