Effect Of Hepatitis B Virus Infection And Antiviral Therapy On Microvascular Invasion Of Hepatocellular Carcinoma

Objective: To study the effects of hepatitis B virus infection and related factors on the formation of microvascular invasion in hepatocellular carcinoma,and further explore the effect of anti-hepatitis B virus treatment on the formation of microvascular invasion of hepatocellular carcinoma.Therefore,it provides certain clinical value for the treatment and prognosis evaluation of hepatitis B virus infection and hepatocellular carcinoma patients.Methods: Retrospective analysis of clinical and pathological data of 660 patients with hepatocellular carcinoma who underwent radical hepatectomy from January 2015 to December 2017 in the Affiliated Hospital of Qingdao University.The main data are collected as follows: 1.Basic data of patients and history of previous hepatitis B virus infection and history of antiviral therapy;2.Results of preoperative serological tests(including hepatitis B virus related tests,tumor related markers and liver function related indicators);Pathological examination results(including: the largest diameter of the tumor,the number of tumors,the degree of tumor differentiation,the integrity of the tumor capsule,cirrhosis and satellite nodules).Statistical analysis was performed on the collected information of 660 patients.The patients were first divided into MVI positive group(M+ group)and MVI negative group(M0 group)according to pathological examination results.Microvascular invasion of hepatocellular carcinoma was obtained by one-way analysis of variance.Possible factors.Then,575 patients with HBsAg positive were divided into MVI positive group(LM+ group)and MVI negative group(LM0 group)according to the pathological examination results.The data of these two groups were based on the positive influencing factors obtained by one-way ANOVA.Data were analyzed by multivariate binary logistic regression to determine independent risk factors or protective factors affecting the formation of microvascular invasion in hepatocellular carcinoma.In addition,according to the presence and severity of MVI,all 660 patients were divided into three groups,namely M0 group,M1 group and M2 group.The relationship between MVI severity and factors related to hepatitis B virus infection wasanalyzed by Kruskal-Wallis test to further verify The results of the study also highlighted the impact of hepatitis B virus infection and anti-hepatitis B virus treatment on the formation of microvascular invasion of hepatocellular carcinoma.Results: This study included 660 patients with hepatocellular carcinoma who underwent radical hepatectomy from January 2015 to December 2017 in the Affiliated Hospital of Qingdao University.There were 534 males and 126 females,accounting for 80.9% of males;the average age was 56 years(range 30-83 years).Among them,there were 272 males and 79 females in the MVI-negative group,with a male ratio of 77.5%;the average age was 57 years(range 31-83 years),262 males and 47 females in the MVI-positive group,males accounted for 84.8%;Aged 55 years(range 30 to 82 years old).The positive rate of hepatitis B virus surface antigen(HBsAg)was 87.1%(575/660),and the incidence of microvascular invasion was 46.8%(309/660).One-way analysis of variance showed that hepatitis B virus surface antigen(HBsAg)-positive,detectable hepatitis B virus DNA(DNA)load and whether more than half a year of antiviral therapy was associated with microvascular invasion.Multivariate binary logistic regression analysis in patients with hepatitis B virus-associated hepatocellular carcinoma showed that the detectable HBV DNA load(OR = 5.33,P < 0.001)was microvascular invasion in the LM+ and MVI-negative groups.Independent risk factors.Antiviral therapy for more than half a year(OR =0.37,P = 0.002)was an independent protective factor for microvascular invasion.Kruskal-Wallis test analysis showed that patients in the M6 ??group had significantly higher HBV infection rates than those in the mild MVI group(M1 group)and the non-microvascular invasion group(M0 group)(94.0).%,P = 0.017),detectable HBV DNA load(> 100 IU/ml)rate(68.3%,P < 0.001)and significantly lower antiviral treatment rate(22.8%,P = 0.012).Conclusion: In patients with hepatocellular carcinoma,hepatitis B virus infection and active replication have an impact on the formation of microvascular invasion.Hepatitis B virus DNA level is an independent risk factor for the formation of microvascular invasion of hepatocellular carcinoma,and anti-hepatitis B virus treatment may have a preventive effect on the formation of microvascular invasion of hepatocellular carcinoma.