| Objective: To explore the expression of fibroblast growth factor receptor-2(FGFR2)and epidermal growth factor receptor(EGFR)in gastric cancer and their correlation with clinicopathological features and prognosis.It provides a new reference for the prognostic evaluation of GC.The correlation between FGFR2 expression and EGFR expression in GC was also analyzed.Methods: Immunohistochemical(IHC)staining was used to detect the expressions of FGFR2 and EGFR in 82 paraffin-embedded specimens of gastric cancer and 20 adjacent normal gastric tissues.We collected the clinical and pathological data of the patients,such as age,sex,differentiation of gastric cancer,depth of invasion,lymph node metastasis,TNM staging,tumor size,vascular invasion,neurological invasion,follow-up data and so on.All the data were sorted out,screened and statistically analyzed by SPSS20.0 software.Results:(1)In 82 paraffin-embedded specimens of gastric cancer,the positive expression rate of FGFR2 was 46/82(56.10%),and the positive expression rate of EGFR was 34/82(41.46%).In 20 normal gastric tissue adjacent to carcinoma,FGFR2 positive expression rate was 4/20(20.00%),and the positive expression rate of EGFR was 2/20(10.00%).The expressions of FGFR2 and EGFR in gastric cancer tissues were significantly higher than that in normal gastric tissues adjacent to the gastric cancer(P<0.05).(2)The positive expression of FGFR2 was significantly correlated with the depth of invasion(χ2=7.134,P=0.008),TNM stage(χ2=15.089,P=0.000),the degree of tumor differentiation(χ2=4.523,P=0.033)and lymph node metastasis(χ2=7.806,P=0.005).There was no significant correlation with sex(χ2=0.348),age(χ2=0.410),tumor size(χ2=0.655),vascular invasion(χ2=0.032)and nerve invasion(χ2=0.110).There was no significant difference between the two groups(P > 0.05).The 2-year survival rate of GC patients with FGFR2 positive expression was 26.09%,and the average survival time was 17 months.the 2-year survival rate of FGFR2 negative expression patients was 50.00%,and their average survival time was 24 months.The survival rate and average survival time of patients with positive FGFR2 expression were significantly lower than those of FGFR2 negative expression patients(χ2=7.323,P<0.01).Cox multivariate survival analysis showed that FGFR2 was an independent prognostic factor in patients with GC.(3)The positive expression of EGFR was significantly correlated with the depth of invasion(χ2=5.303,P=0.021),TNM stage(χ2=4.181,P=0.041),lymph node metastasis(χ2=4.225,P=0.040)and vascular invasion(χ2=8.073,P=0.004).There was no significant correlation between EGFR positive expression with other clinicopathological parameters(P>0.05),such as gender(χ2=0.000),age(χ2=0.505),tumor size(χ2=0.175),tumor differentiation(χ2=0.365),and neurological invasion(χ2=0.432).The 2-year survival rate of GC patients with EGFR positive expression was 23.53%,and the average survival time was 18 months.The 2-year survival rate of EGFR negative expression patients was 45.83%,and the average survival time was 21 months.The survival rate and average survival time of GC patients with EGFR positive expression were significantly lower than those of EGFR negative expression patients(χ2=4.306,P < 0.05).(4)Spearman correlation analysis showed that FGFR2 expression was negatively correlated with EGFR expression,but there was no statistically significant difference(P>0.05).Conclusion:(1)The positive expression rates of FGFR2 and EGFR in GC are higher than those in normal gastric tissues.(2)The positive expression of FGFR2 and EGFR can promote the occurrence and progression of GC,which is closely related to the poor prognosis of GC.(3)FGFR2 expression was negatively correlated with EGFR expression,but there was no statistically significant difference. |
PDF Full Text Request