Influence Of Different Probiotic On Lipid Metabolism Of High Fat Diet Induced Obese Mice And Changes Of Intestinal Flora In Some Mice

Objective: To study the influence of different probiotic intervention on lipid metabolism and LPS of high fat diet induce obese mice at different stages.Compared the changes of intestinal flora between the normal diet group and the high-fat diet group mice.To analyze the dynamic changes in the intestinal flora during the life cycle between the two group.Methods: One hundred and eight male C57BL/6 mice were randomly assigned t o a control group,a high-fat diet group and high-fat diet+probiotic intervention gr oups(choose bifidobacterium infantis groups and clostridium butyricum,divided in to high and low dose group),eighteen mice in each group,fed for 5 weeks.On the first,third and fifth weekend of breeding,six mice in each group were killed,blo od lipid and LPS were measured.To study the composition and characteristics of intestinal flora in the normal diet group and the high-fat diet group mice by ill umina amplicon sequencing of 16 Sr DNA tag.Results: Compared with control group and high-fat diet group+probiotic intervent ion groups,the body weight of obesity mice was significantly higher than that gr oups(P<0.05).The body weight of the high dose probiotic intervention mice were lower than the low dose group(P<0.05).TC,TG,LDL,LPS in high-fat diet group were significantly higher than the control group(P<0.05).The probiotic intervention groups had significantly lower levels of TC,TG,LDL,LPS than the high-fat diet group(P<0.05).HDL in high-fat diet group were significantly lower than the contr ol group(P<0.05).The probiotic intervention groups dramatically increased HDL le vels compared with the high-fat diet group(P<0.05).At the phylum,the number of Bacteroidetes showed a parabolic trend from high to low in the intestinal flora of mice in the normal-fat diet group.Deferribacteres was opposite.The number of Firmicutes showed a parabolic trend from high to low in the intestinal flora of mice in the high-fat diet group.Bacteroidetes was opposite.At different stages the number of Lactobacillus was significantly higher in the normal-fat diet group th an the high-fat diet group.Conclusion: Probiotic intervention may improve the obesity caused by high-fat d iet,and reduce the content of LPS.Bifidobacterium infantis and clostridium butyric um has different effect for obese individuals in different dose.At different stages of the life cycle of mice,the dominant bacteria in the intestinal flora change d ynamically,and the change of the dominant bacteria in the intestinal flora is clo sely related to the occurrence of obesity.