Experimental Study On Protective Effects Of Citrus Flavonoids On Ovary And Blood Vessels In Rats

Objective: To investigate the protective effect of citrus flavonoids on cyclophosphamide-induced ovarian injury and carrageenan-induced vascular injury in rats,and to provide an important scientific basis for further study on the medicinal value of citrus flavonoids and its comprehensive development and application.Methods : The first part: 50 SPF SD rats(female,not pregnant),adapted for one week,were randomly divided into 5 groups,10 rats/group,respectively,low-dose citrus flavonoids,high dose of citrus flavonoids.Group,Kuntai capsule group,model control group,blank control group;the first 4 groups were induced by cyclophosphamide for the first time after 50 mg/kg intraperitoneal injection,and then induced by intraperitoneal injection(8 mg/kg)for 14 days.The blank group was intraperitoneally injected with the same amount of normal saline.After the model was completed,the citrus flavonoid low dose group(80 mg/kg citrus flavonoids)was intragastrically administered,the citrus flavonoid high dose group(160 mg/kg citrus flavonoids)was intragastrically administered,and the Kuntai capsule group(1.2 g/kg Kuntai)was intragastrically administered.The blank control group and the model control group(10 ml/kg normal saline)were intragastrically administered,and each group was continuously intragastrically administered for 3 weeks.On the first day after the end of the gavage,the estradiol(E2)in the serum of the rats was detected by chemiluminescence method.Progesterone(P)and anti-Muller test tube hormone(AMH)levels,RT-qPCR quantitative detection of bone morphogenetic protein 15(BMP15)and growth differentiation factor 9(GDF9)mRNA expression and HE staining observed ovarian pathological morphology.The second part: 40 SD rats(male and female),10/group(male and female),were randomly divided into 4 groups: blank control group,model control group,citrus flavonoid low dose group and citrus flavonoid high dose group.Low-dose citrus flavonoids(80mg/kg),high-dose citrus flavonoids(160mg/kg)were administered to the stomach,and the blank control group and the model control group were administrated with the same amount of normal saline(10ml/kg)for 8 days.In addition to the blank control group,the remaining 3 groups were induced by intraperitoneal injection of carrageenan(20 mg/kg dose)on the 8th day of intragastric administration.After 24 hours,the length of the black tail was observed and taken,and thepathological morphology of the blood vessels was observed by HE staining.Serum miRNA126 expression was detected by RT-qPCR,serum protein C and protein S levels were detected by ELISA,and serum TH17 levels were detected by flow cytometry.Results: 1.Compared with cyclophosphamide-induced ovarian injury model control rats,estradiol,progesterone,AMH levels and rat ovaries in serum of low dose(80mg/kg)and high dose(160 mg/kg)rats The expression of BMP15 and GDF9 in tissues was significantly increased(P<0.05),and HE staining showed the presence of follicles at all levels,with little or no necrotic follicles.2.Compared with cross-brassica oleracea induced vascular injury model rats,miRNA126,protein C and protein S levels in the serum of low-dose and high-dose citrus flavonoids rats were significantly increased,and the expression of TH17 was down-regulated,and the length of the black tail was significantly shortened.Conclusion: 1.Citrus flavonoids play a role in repairing damaged ovaries by maintaining the growth and maturation of follicles,up-regulating the expression of BMP15 and GDF9,and balancing the levels of estrogen and progesterone.2.Citrus flavonoids can protect against vascular endothelial injury by regulating the expression of miRNA126,protein C,protein S and TH17,inhibiting intravascular thrombosis and reducing inflammatory response.