Study On The Chemical Constituents And Inhibitory Effects On HIF-1α Activation Of Rhodiola Sachalinensis A.Bor.

In this study,twenty-two compounds were obtained from ethyl acetate and n-butanol fractions of the rhizomes of Rhodiola sachalinensis A.Bor.by normal and reverse phase silica gel column chromatography,macroreticular resin,Sephadex LH-20 and semi-preparative HPLC.The isolated compounds were identified as SudanⅢ(1),pentatriacontane(2),ndecane(3),dibutyl phthalate(4),2,6-dimethoxy-4-hydroxyacetophenone(5),,4-dihydroxy--methoxyphenylethanone(6),emodin(7),2(R)-26-[(2E)-3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl]oy]-2,3-dihydroxypropylester(8),methyl gallate(9),tyrosol(10),caffeic acid(11),gallic acid(12),β-arbutin(13),2-methoxyhydroquinone(14),kaempferol(15),p-hydroxybenzoic acid(16),protocatechuic acid(17),4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone(18),2,6-dimethoxyacetopheonone-4-O-(β-D-glucopyranosyl)acetophenone(19),rutin(20),3’-methoxyl Luteolin-7-O-β-D-glucoside(21)and galuteolin(22)on the basis of NMR and MS spectroscopic analysis.Among them,compounds 1~3、5~9、14、18、19、21、22 were isolated from the plant for the first time.The isolated compounds were evaluated for their inhibitory effects on HIF-lce activation in Hep3B cells,and cell viability was measured by MTT assay.As a result,compounds 6、12、15、17、18 exhibited inhibitory activities on HIF-la transcription,with showing no obvious cytotoxicity at the corresponding concentration of action.Among them,compounds 12,15,and 18 exhibited dose-dependent effect on HIF-1α activation.