Study On The Construction And Mechanism Of Biopharmaceutical Small Molecule Detection System

At present,the use of quantum dots in the detection of small molecules of biological drugs in the market is basically blank..Quantum dots are a kind of semiconductor nanomaterials with excellent performance.They have been widely used in analytical detection and biomedicine because of their wide excitation spectrum,good biocompatibility,and easy modification.Hot spot.Due to the introduction of new elements,doped quantum dots make the mother’s performance better and become a new material star.However,most of the studies on doping quantum dots are based on their fluorescence properties.There are still few studies on their phosphorescence properties.Compared with fluorescence,since the phosphor dots of the quantum dots have a proper delay and a long lifetime,the self-fluorescence and stray light interference can be avoided in the material analysis process,and the analysis is more stable and reliable.In addition,phosphorescence is more difficult to generate than fluorescence,so the specificity of the phosphorescence detection is better,and the sample to be tested does not need complicated pretreatment,which greatly simplifies the detection process of the target,which is a Room-Temperature Phosphorscence(RTP)method.Conducting material identification analysis brings new opportunities.Therefore,the phosphorescence efficiency of this study highlights 3-mercaptopropionic acid(MPA)encapsulated Mn-doped ZnS QDs(Mn:ZnS QDs),using their superior phosphorescent optical properties,and fully combining with target analysis.The molecular structure and chemical properties of the compounds were investigated and established.The room temperature phosphorescence analysis system for the simple,sensitive and highly efficient detection of palmatine hydrochloride,jatrorrhizine hydrochloride and levofloxacin was studied.The full text is divided into five parts,the main contents are as follows:(1)MPA-coated Mn:ZnS QDs were prepared and characterized by the aqueous phase method.In combination with the phosphorescence efficiency of the quantum dots and the quenching of phosphorescence by palmatine hydrochloride,a phosphorescence method for the high-performance analysis of palmatine hydrochloride was proposed.In the linear range of0.75 μmol/L to 30 μmol/L,the concentration of palmatine and the quantum dot luminous intensity have a certain regularity,the correlation coefficient is 0.996,and the detection limitis 0.35 μmol/L.The detection is mainly based on the positively charged palmatine hydrochloride adsorbed on the quantum dot body surface by electrostatic action,and further quenches the phosphorescence of the quantum dot by electron transfer effect.The method does not require complicated pretreatment in the actual determination of palmatine hydrochloride,and the analytical performance is excellent.(2)Based on the regular effect of jatrorrhizine hydrochloride on phosphorescence of Mn:ZnS QDs,a method for the phosphorescence quenching of jatrorrhizine hydrochloride in trace biological fluids and actual pharmaceuticals was proposed.The process was mainly based on califorine and QDs.Phosphorescence of quantum dots was effectively quenched by static attraction between them,and the determination of jatrorrhizine hydrochloride was realized.Under optimal conditions,the response range of Mn:ZnS QDs to jatrorrhizine hydrochloride ranged from 1 to 92umol/L,the correlation coefficient was 0.995,and the detection limit was 0.39umol/L.This method was applied to real-time samples of the hydrochloride Alkaline detection,the results are satisfactory.(3)Based on the effect of levofloxacin on RTP quenching of Mn:ZnS QDs,a new phosphorescence method for the determination of levofloxacin was established.The linear range of the method is 0.5 μmol/L ~100 μmol/L,the correlation coefficient is 0.995,and the detection limit is 0.35.Μmol/L.The trace effect of levofloxacin in this method is excellent,and the process is simple.In addition,the selectivity of detection is stronger because phosphorescence is more difficult to generate than fluorescence.In conclusion,based on the superior RTP performance of 3-MPA-encapsulated Mn:ZnS QDs,the regularity of the phosphorescence efficiency of the QDs was quenched by the analytes,a trace analysis method for three commonly used drugs was established,and the effects of each system were explored in detail.mechanism.The establishment of the system overcomes some of the deficiencies of the existing methods,solves the difficulty of the simple and highly effective determination of drugs at the present stage,and provides an alternative method for clinical testing of related drugs that is convenient,low-cost,and highly efficient.