Based On Camellia Flavonoids For Diabetes In Mice Pharmacodynamic Study Of The Model

Objective: To study the efficacy and mechanism of total flavones of Camellia japonica(TFC)on diabetic mice and animal models.The model of type 1 and type 2 diabetes mellitus was established by STZ induction method,and the intervention of total flavonoids of Camellia japonica on serum inflammatory factors,lipid metabolism and oxidative stress in diabetic model was investigated.The effect of total flavonoids of Camellia sinensis on diabetes and detailed mechanism were comprehensively studied.Methods:a diabetic mouse model was established by intraperitoneal injection of streptozotocin(STZ)0.15g/kg for 1 days.According to the blood sugar value,the groups were divided into model group,metformin group,large,medium and small dose group of total flavonoids of Camellia,each group was given the corresponding drug every day.The model group and the blank group were given the same volume of normal saline.One time a day for 31 days,the dynamic blood glucose,serum glycosylated serum protein(GSP)content and liver sugar were measured in each group.(Lg)the original content.The pancreas and kidney were fixed by 4% Formaldehyde Solution,and the pathological changes were observed by HE staining.A diabetic rat model was established by intraperitoneal injection of streptozotocin(STZ)0.06g/kg for 1 days.According to the blood sugar value into the model group,metformin group,the large,medium and small dose group of total flavonoids of Camellia,each group was given the corresponding medicine every day.The model group and the blank group were given the same volume of normal saline each day for 31 days.The dynamic blood glucose values of each group were measured,and the diet,drinking water amount and urine were measured on the same day.Quantity and weight,serum glycosylated serum protein(GSP),insulin(INS),leptin(LEP),insulin antibody(ICA),total antioxidant capacity(TAO-C),glutathione peroxidase(GSH-PX),tumor necrosis factor(TNF-α),interleukins 1-beta(IL-1β),interleukins(IL-6),total cholesterol(TC),total cholesterol(TC),and total cholesterol(TC),triglyceride(TG)content and whole blood viscosity.The pancreas,kidney,liver,thymus and spleen were fixed with 4% Formaldehyde Solution,and the pathological changes were observed by HE staining.The rats were fed with high fat and high sugar diet for 4 weeks,and STZ 0.09g/kg was injected intraperitoneally to establish a diabetic mouse model of 2.According to the blood sugar value into the model group,metformin group,the large,medium and small dose group of total flavonoids of Camellia,each group was given the corresponding medicine every day.The model group and the blank group were given the same volume of normal saline each day for 31 days.The dynamic blood glucose values of each group were measured,and the diet,drinking water amount and urine were measured on the same day.Quantity and weight,serum glycosylated serum protein(GSP),insulin(INS),leptin(LEP),insulin antibody(ICA),total antioxidant capacity(TAO-C),glutathione peroxidase(GSH-PX),tumor necrosis factor(TNF-α),interleukins 1-beta(IL-1β),interleukins-6(IL-6),total cholesterol(TC),total cholesterol(TC),and total cholesterol(TC).,triglyceride(TG)content and whole blood viscosity.The pancreas,kidney,liver,thymus and spleen were fixed by 4% Formaldehyde Solution,and the pathological changes were observed by HE staining.Result:The animal model of type 1 diabetic mice was successfully replicated from the animal blood glucose value and polypodities,polydipsia and polyuria.Compared with the model group,the metformin group and the TFC dose groups significantly decreased the dynamic blood glucose and GSP content in the serum of the model animals,increased the content of Lg,and improved the pathological changes of the pancreas,kidney and heart of the model animals.Change.The animal model of type 1 diabetic rats was successfully replicated from the animal blood glucose value and polypodities,polypodities and polyuria.Compared with the model group,the metformin group and TFC dose groups could significantly reduce the diet,drinking water,urine volume,decrease the weight loss of animals,reduce the serum blood glucose,GSP,LEP,ICA,TNF-α,IL-1.The content of beta,IL-6,TC,TG and blood viscosity increased the value of INS,TAO-C and GSH-PX in the serum,and improved the pathological changes of the pancreas,kidney,liver,spleen and thymus of the model animals.The animal model of type 2 diabetic mice was successfully replicated from the animal blood glucose value and polypodities,polypodities and polyuria.Compared with the model group,the metformin group and TFC dose groups could significantly reduce the diet,drinking water and urine volume,and inhibit the weight loss of model animals.The serum levels of blood glucose,ICA,TNF-α,IL-1β,CRP,LEP,TG,TC and LDL-C were reduced,and HDL-C,INS,ADPN and Lg values were increased in the serum,and the pathological changes of the pancreas,kidney,liver,spleen and thymus of the model animals were improved.Results: All animal models of diabetes have been successfully replicated.Model animals present serum INS,LEP(type I),T-AOC,GSH-PX,TNF-α,IL-1β,IL-6,TC,TG,HDL-C The level of indicators such as serum ICA,GSP,LEP(type II),LDL-C,and whole blood viscosity,and other indicators of the level of elevated levels,for the above animal models of diabetes present corresponding changes in biochemical indicators,the total flavonoids of camellia can be significantly increased Serum levels of INS,LEP(type I),T-AOC,GSH-PX,TNF-α,IL-1β,IL-6,TC,TG,HDL-C,etc.,reduced serum ICA,GSP,and LEP(type II)),LDL-C and whole blood viscosity of each degree of cut and other indicators to improve the corresponding changes in biochemical indicators of diabetic animal models.The animal model of diabetes will show obvious pathological symptoms of high dietary intake,water intake,and urine output,as well as weight loss.The results show that the total flavonoids of Camellia can significantly reduce dietary intake and drinking water for the above pathological symptoms.The amount and urine level gradually increase the weight value.In response to the pathological changes in animal models of diabetes,the total flavonoids of Camellia can improve the pathological lesions of pancreas,kidney,liver,thymus,spleen and heart in diabetic animal models.Conclusion:According to the effects of total flavonoids of Camellia japonica on the model 1 and type 2 models of diabetes in mice and mice,the results showed that the blood sugar of the animal model animal was increased and the symptoms were less than 3 or more,which showed that the model was successful.Total flavonoids of Camellia japonica can improve the symptoms of more than three of the model animals,inhibit the occurrence of inflammatory reactions,reduce the secretion and expression of inflammatory factors(TNF-α,IL-1β,CRP,IL-6),inhibit the interference of insulin signal transduction,regulate the disturbance of glucose metabolism,increase the content of TAO-C and GSH-PX,enhance the antioxidant capacity of the body,and reduce the lipid peroxidation of the body.Oxide accumulation,inhibit the occurrence of oxidative stress,reduce the value of TG,TC,LDL-C,increase the content of HDL-C and ADPN,improve the level of LEP,regulate the metabolic disorder of blood lipid,improve the level of blood lipid,and have significant effect on the diabetes model.