The Impact Of Total Flavonoids In Cephalanoplos On Mouse And Rats Diabetic Model

Objective: This study is to investigate the therapeutic effect of thistle total flavone extracts on diabetic mice and rats models by measuring the indexes of glucose metabolism, lipid metabolic, oxidative stress and observing morphological changes of pancreas.We try to explain the therapy effect and function characteristics of thistle total flavone extracts on diabetic mice and rats models.Methods: The method of intraperitoneal injection alloxan was used to establish the hyperglycemia mice models. Animals were feed for three days with normal diet and then treated with alloxan(5%, 200mg/kg) according to weight by i.p.. After 72 hours,measuring blood glucose value of the tail vein blood. Mice that blood glucose value are greater than 11.1mmol/L were randomly divided into 5 groups: the model group,metformin suspension group, thistle total flavone extracts suspensions of high,medium and low dose groups, 12 mice per group. Blank group and the control group fed the same volume 0.1% CMC. All animals were feed for 7 days and hungered 12 hours before the last administration. Two hours after the last delivery, measure blood glucose. Weighing 80 mg liver, measured glycogen. Taking pancreatic for Pathology section.The method of tile intravenous injection Streptozotocin(STZ) was used to establish the diabetic mouse models. Animals were feed for three days with normal diet and then treated with STZ(using citrate buffer solution prepared 10mg/m L STZ,80mg/kg) according to weight by i.v.. After 7 days, hungered 12 hours before,measuring blood glucose value of the tail vein blood. Mice that blood glucose value are greater in 16.7mg/L-21.0mmol/L were randomly divided into 5 groups: the model group, metformin suspension group, thistle total flavone extracts suspensions of high, medium and low dose groups, 12 mice per group. Blank group and the control group fed the same volume 0.1% CMC. All animals were feed for 30 daysand measuring blood glucose value of the tail vein blood at 10,20,30 day after hungered 12 hours.At the 31 day,hungered 12 hours before the last administration.Two hours after the last delivery, measure blood glucose;(TG), cholesterol(TC),serum insulin(Ins), insulin resistance,glycosylated serum protein(GSP), reactive oxygen species(ROS), weighing 80 mg liver, measured glycogen. Taking pancreatic and liver for pathology section.The method of tile intravenous injection Streptozotocin(STZ) was used to establish the diabetic rats models. Animals were feed for three days with normal diet and then treated with STZ(using citrate buffer solution prepared 10mg/m L STZ, 40mg/kg)according to weight by i.v.. After 7 days, hungered 12 hours before,measuring blood glucose value of the tail vein blood. Mice that blood glucose value are greater in16.7mg/L-21.0mmol/L were randomly divided into 5 groups: the model group,metformin suspension group, thistle total flavone extracts suspensions of high,medium and low dose groups, 14 mice per group. Blank group and the control group fed the same volume 0.1% CMC. All animals were feed for 30 days and measuring blood glucose value of the tail vein blood at 10,20,30 day after hungered 12 hours.At the 31 day,hungered 12 hours before the last administration. Two hours after the last delivery, measure blood glucose; triglyceride(TG), cholesterol(TC), serum insulin(Ins), insulin resistance, glycosylated serum protein(GSP), Hydrogen Peroxide(H2O2), Fungal catalase(CAT), HDL cholesterol, LDLcholesterol, b-cell lymphoma factor 2(Bcl-2), Bcl-2-associated x protein(BAX), reactive oxygen species(ROS), superoxide dismutase(SOD), malonic dialdehyde(MDA), NO level and NOS activity, Taking pancreatic and liver for pathology section.Results:Hyperglycemia models of mouse built by alloxan are copied successfully. Com pared with the model groups, both metformin and thistle total flavone extracts can decrease the blood glucose value(P<0.05) and increase glycogen content of hyperglycemia(P<0.01), and inhibit gluconeogenesis and maintain blood glu cose levels, improving the pathological changes of pancreas.Hyperglycemia models are copied successfully built by STZ are copied successfully.Compared with the model groups, both metformin and thistle total flavone extracts can decrease the blood glucose value after 10 days(P<0.05), and increase glycogen content of hyperglycemia, glycated serum protein content, higher content of hepatic glycogen, improve the pathological changes of pancreas and kidney. Decrease thecontent of H2O2 and the lever of ROS(P<0.01), improving the pathological changes of pancreas.Hyperglycemia models are copied successfully built by STZ are copied successfully.Compared with the model groups, both metformin and thistle total flavone extracts can decrease the blood glucose value after 10 days(P<0.05), and increase glycogen content of hyperglycemia(P<0.01), glycated serum protein conten(P<0.01)t, higher content of hepatic glycogen(P<0.01), improve the pathological changes of pancreas and kidney(P<0.01). Decrease the content of H2O2 and the lever of ROS(P<0.01),improving the pathological changes of pancreas. Reduce triglyceride and cholesterol levels(P<0.01)(P<0.01), rises high oxide dismutase(SOD) enzyme activity(P<0.01),reduce the levels of nitric oxide(NO)(P<0.01), reduce the levels of nitric oxide synthase(NOS) activity(P<0.05), Decrease the content of LDL(P<0.05) cholesterol and increase the content of HDL cholesterol(P<0.01). Improve the lever of serum insulin(Ins)(P<0.01), glycosylated serum protein(GSP)(P<0.01), fungal catalase(CAT)(P<0.01), reduce the lever of insulin resistance and the content of Hydrogen Peroxide(H2O2)(P<0.01), reduce the content of Bcl-2 and impover the BAX(P<0.01). Improve the pancreas, liver changes.Conclusion:Total flavonoids in Herba cirsii extracts on diabeticmodel rats and mice have very good protection. Total flavonoids in Herba cirsii extracts to reduce blood glucose levels and glycosylated serum protein, lowertriglycerides, cholesterol, high density lipoprotein, lowdensity lipoprotein, high glycogen content. So as to improve the lipid metabolism of the organism; promote the secretion of insulin and glycogen synthesis, increase of GSH-PX, H2O2 content, enhanced the activityof SOD, Bcl-2,ROS, reducing the content of NO and the CAT, reduced BAX, NOS activity, lower serum MDA levels, To improve the degree of oxidative stress in the body.Pathological changes of pancreas can be improved. Integrated play a role in protecting diabetic.