Study On Bone Binding Of Titanium Alloy-antibacterial Peptide KSL

Research objective: In order to further improve the success rate of titanium alloy implant implantation,the experiment of micro arc oxidation coating on titanium alloy implant(Microarc Oxidation,referred to as MAO),with coupling agent silane(Silane Coupling Agent,referred to as SCA)as the further loading of antimicrobial peptid KSL,namely the MAO-SCA-KSL composite membrane is formed to achieve the effects of antibiosis and bone growth.Research method: The titanium alloy implant with microarc oxide film layer(group A),microarc oxidation-silane composite membrane layer(group B)and microarc oxidation-silaneantibacterial peptide KSL composite membrane layer(group C)are set up respectively.The 3 groups of implants are implanted into the mandible of rabbits respectively,and then inject the immunofluorescent labeling after random tagged grouping.Kill the corresponding rabbits after 2weeks,4weeks and 8 weeks respectively,and carry out CBCT observation,scanning electron microscope and energy spectrum analysis,laser confocal microscope observation,acid fuchsinmethylene blue staining to detect the synostosis strength of implant-bone interface,and a torsion test is carried out to further detect the strength of the implant synostosis.Result analysis: There is no osteonecrosis or soft tissue purulent infection occurred in 3 groups of implant-bone interface.The inspection results of CBCT shows that with the prolongation of implantation time,the low density shadow around the implant,especially at the implant bone interface,gradually decreased,and the low density shadow of the antibacterial peptide group is the smallest,which shows statistical significance observation results of laser confocal microscope: In the same time point,the of fluorescence bands continuity of antibacterial peptide group is best,the fluorescence intensity is strongest,and the band width is widest;At 4th week,the new bone mineralization deposition rate of antibacterial peptide group(4.8±0.28μm/d)is significantly higher than that of A(3.7±0.05μm/d)and B(3.9±0.15μm/d)two group(P<0.05),which shows statistical significance.Results of scanning electron microscope and energy spectrum analysis show that at 8th week after implantation,some new bone in 3 groups has been transformed into mature bone.The antimicrobial peptide group shows the most obvious performance.The surface scanning element analysis on implant-bone interface shows that the content of Ca and P elements on the implant-bone interface is highest at 4th week after implantation,and the osteogenesis of group C at 4th week is most active,namely the antibacterial peptide group can promote early osteogenesis.Results of acid fuchsin-methylene blue staining show that the synostosis index of antibacterial peptide group(76.85%±4%)is significantly higher than that of MAO(42.39%±2.53%)and silane(47.29%±1.2%)(P<0.05),which shows statistical significance.At 8th week after implantation,the torque value of antibacterial peptide group(53.77±1.54N·cm)is significantly higher than that of MAO(26.9±1.28N·cm)and silane(39.30±0.82N·cm)(P<0.05),which shows statistical significance,indicating that the osseointegration strength of antibacterial peptide group is better.Conclusion: MAO-SCL-KSL composite membrane has a higher synostosis ability,and the bone healing time is shortened,which can promote early bone formation and improve the synostosis strength.