Bone Compatibility Study On Titanium Alloy Implants With Micro-arc Oxidation By Silane Coupling Loading Of Antimicrobial Peptide Pac525 Coating

Objective In order to improve the biocompatibility of the titanium alloy implant,we build on the surface of titanium alloy implants micro-arc oxidation-silane-antibacterial peptide Pac525 coating.And implanted in the mandibular of the rabbit,study the coating in bone combined with the behavior of animals.Methods Micro-arc oxidation processing of titanium alloy implants to form the surface porous ceramic layer structure.Connection after activation treatment with alkali heat method silane coupling agent KH-550.Finally loaded antibacterial peptide Pac525 coating.With MAO implant for group A,Micro-arc oxidation-silane implant for group B,Micro-arc oxidation-silane-antibacterial peptide Pac525 implant for group C.Implanted in rabbit mandible,points 2,4,8 three cycles.Using cone beam CT(CBCT),laser confocal microscope(CLSM),scanning electron microscope(SEM)and energy spectrum(EDS),torsion experimental methods such as observation detection of implant and bone interface combined with the situation.Results CBCT shows 4 weeks when started to higher density images around implant,8 weeks of high density range image expanded,group C is better than that of group B and group A.Laser confocal microscope shows that 4weeks the calcein show stronger light,alizarin red are widely distributed,8 weeks,double fluorescent tags expanded.The Bone mineralization deposition rate shows that group C is better than that of group B and group A,group B is better than that of group A and statistically significant(P?0.001).SEM and elemental analysis shows that eight weeks form of wide belt is superior to bone fusion narrow fusion with 2,4 weeks,and 4 weeks active element in the bone fusion zone distribution is better than two weeks.Torsion experiment shows that group C is better than that of group B and group A,group B is better than that of group A and statistically significant(P?0.01).The surface morphology of the implant which after the torsion experiment can be seen screw thread grooves in group C attached to A large number of bone tissue,significantly more than group A and group B.Conclusions After micro-arc oxidation treatment of titanium implant loaded by silane coupling way of 0.75mg/ml antimicrobial peptide Pac525 coating,can promote the combination of bone,to enhance the effect of early implant stability.