Identification And Discovery Of Genetic Risk Factors For Coronary Artery Disease In The Han Population Of Central China

Objective : Coronary artery disease(CAD)is the most common disease of causing death in the world.It is a complex disease caused by environmental factors and genetic factors.The genetic factors account for 40% to 60%.Recently,Genome Wide Association Study(GWAS)found six CAD susceptibility single nucleotide polymorphism(SNP)loci in the European population and South Asian population.The purpose of this study is to verify the association of SNP rs1801251 with CAD in the Han population of Central China.Methods: This study was performed in 2759 CAD cases(1820 males and 939 females,aged 65.61±12.54 years old)and 1601 controls(926 males and 675 females,aged 63.10±9.37 years old).All DNA samples were genotyped by High Resolution Melt(HRM)method and 30 DNA samples were randomly selected for direct sequencing to determine the accuracy of the genotyping results.Finally,we performed allelic association analysis and genotypic association analysis on typing results.Results: The results of allelic association analysis between rs1801251 and CAD in the Han population of Central China showed that there was no significant difference in allele frequencies between CAD group and control group(Pobs=0.28,Padj=0.60).The genotypic association analysis of SNP rs1801251 and CAD in Han population of Central China showed that there was no statistical significance under three different genetic models of additive,dominant and recessive(Pobs>0.05,Padj>0.05).The results of allelic association analysis and genotype association analysis in male,hypertension and non-hypertension population respectively were all not statistically significant(Pobs > 0.05,Padj > 0.05).In female population,the allelic association analysis showed statistical significance(Padj=0.03,Padj=0.05),and the results of genotype association analysis under three models were not statistically significant(Padj>0.05,Padj>0.05).Conclusion: No significant association between SNP rs1801251 and CAD was found in the Han population of Central China,which was a CAD susceptibility SNP locus in the European population and South Asian population.But SNP rs1801251 was associated with CAD in female population.