Functional Micro/Nano-interface Based Innovative Approaches To The Detection Of Circulating Tumor Cell

As one of the primary leading causes of death,cancer catches researchers’ eye all the time.Developing diagnostic techniques for cancer is always the top priority.Traditional diagnostic approaches,including tissue biopsy,imageological diagnosis and serum tumour markers detection,were limited by their low sensitivity,poor robustness and harmful method,which can not meet the demands of future cancer diagnosis.Nowadays,detection of circulating tumor cells(CTCs)becomes a new star in the field of cancer diagnosis because of its numerous advantages.CTCs are tumor cells that escape from the primary tumor and shed into the bloodstream as tumors grow.Containing the vast majority information of solid tumor,CTCs are able to help doctors have a better understand about the patient without invasion.However,CTC detection is still a hard work for CTC detection due to the extraordinarily low aomunt of CTC.Microfluidic chips have virtues such as quick reflexes,high effiency,high throughput,low sample waste and good operation,and are applicable to CTC detection.In this thesis,deterministic lateral displacement(DLD)principle is integrated with target affinity recognition in one microfluidic chip to implement size-dictated immunocapture,which enables high performance detection for CTCs.For different purposes on CTC enrichment and analysis,we constructed different kinds of functional Micro/Nano-interface.In the first job,we modified antibody on micropillars that specifically recognizes CTCs in chip channel.With the help of strong binding force between antibody and CTC,prostate cancer cells were successfully captured at a high recovery rate and authentic CTCs were successfully obtained from prostate cancer patients’ blood.As to the second work,we utilized gold nanoparticles to highly enrich aptamers in restricted space,so that it enhanced the affinity of aptamer to CTC.Beyond this,aptamers on gold nanoparticle can be replaced to GSH molecules.Through this process,CTCs captured on chip were release leniently without injury.Moreover,captured CTC could be cultured or further analyzed such as cell sequencing.It provides reasearchers a befitting approach to CTC’s deep analysis.