The Research Of Relationship Between The Human Gut Microbioa And The Lipid-lowering Effects Of Rosuvastatin

Objective:To study the relationship between the Lipid-lowering effect of rosuvastatin and lipid microbial population in patients with hyperlipidemia,and to analyze the relationship between hyperlipidemia and hypolipidemia in patients with hyperlipidemia after hematopitrazine Microbial populations of the differences,for the clinical use of rosuvastatin hypolipidemic and prevention of cardiovascular disease events to provide new clues.Methods:Sixty-four patients with thalidomide hypolipidemic were selected,including 45 cases of male patients and 19 cases of female patients,from 55 to 75 years old.Fecal were stored at-80°immediately after collecting.Then isolated the whole DNA according to the protocols of the Stool DNA kit,and amplified the variable regions V3-V4 of the 16 S rRNA gene.The PCR products were sequenced in the platform of Illuminate Hiseq PE250.At last,the data were profiled by building a library and multivariate statistics.result:1.clinical laboratory examination indicators of lipid levels TG and LDL-C levels in the two groups were significantly lower than those in the low-fat group（P <0.01）,and the LDL-C ratio in the low-fat group was 58.5 %,Whilethe high fat group was only 31.2%;low fat group TC reduction of 41.9%,while the high fat group was only 26.6%.1.use of rosuvastatin in patients with hypolipidemic hyperlipidemia and low blood lipid group intestinal microbial population of bacteria In phylum level,the abundance of the Firmicutes,Firmicutes / Bacteroidetes in the low-fat group was significantly higher than that in the high-fat group,and Actinobacteria and Cyanobacteria was significantly lower than the abundance of high-fat group.In family level,the abundance of Ruminococcaceae,Lachnospiraceae,Veillonellaceae,Christensenellaceae,Lactobacillaceae,Clostridiaceae₁,Coriobacteriaceae and Verrucomicrobiaceae decreased in low-fat compared with high-fat group.The abundance of Bacteroidaceae,Prevotellaceae,Pseudomonadaceae,Rikenellaceae,Streptococcaceae,Acidaminococcaceae,Enterobacteriaceae,Bifidobacteriaceae decreased in high-fat compared with low-fat group.In genus level,the abundance of Ruminococcaceae_UCG-014,Ruminococcaceae_UCG₀02,Dialister,Pseudobutyrivibrio,Subdoligranulum,Christensenellaceae_R7group,Blautia,Collinsella,Citrobacter,Clostridium_sensu_stricto₁,Eubacterium_hallii_group,Coprococcus₃ decreased in low-fat compared with high-fat group.The abundance of Bacteroides,Parabacteroides,Veillonella,Escherichia_Shigella,Prevotella₉ decreased in high-fat compared with low-fat group.3.The alteration of intestinal flora between high-fat group and low-fat group on gender In phylum level,the two groups of female patients,low-fat group than the high-fat group of Firmicutes increased significantly and Saccharibacteria significantly reduced.Differences in male patients are also mainly reflected in the Firmicutes.In family level,the diversity of intestinal microflora in female patients with low fat group was significantly higher than that in male patients.Lactoseaceae,Lacnospiraceae,Peptostreptococcaceae,Family_XIII,Defluviitaleaceae,and probiotic Lactobacillus inthe low-fat group were significantly higher in the intestinal tissues In the high-fat group;and in the Bacteroides,Prevobacterae and Enterobacteriaceae is lower than the high-fat group,and mostly of them are opportunistic pathogens.4.The alteration of intestinal flora between high-fat group and low-fat group in the age Among the patients over 65 years of age,the phylum Fusobacteria was higher than that in the low-fat group,while the Bacteroidaceae and the family Peptostreptococcaceae was more in the high-fat group.Among the patients under 65 years of age,family Lachnospiraceae,Erysipelotrichaceae,Desulfovibrionaceae and Clostridiales_vadinBB60_group were significantly higher in the low-fat group than in the high-fat group.Conclusion:1.Intestinal flora in low-fat group exist obviously differences with the high-fat group.2.Intestinal flora in low-fat group on the gender exist obviously differences with the high-fatgroup.3.Intestinal flora in low-fat group on the ages exist obviously differences with the high-fat group.