Changes Of Metabolic Profiling,in Flammation And Gland Histopathology In Mouse Model With Parkinson’s Disease

Objective: To investigate the changes of metabolic profiles,behavior,adrenal gland histology,activated microglia in Parkinson’s disease model.Methods: 20 male C57 BL mice were divided into model group and control group randomly.The mice received bake hypodermic injections of MPTP twice a week for 5 consecutive weeks.The physical behavior(pole test,traction test,swim test)of two group animals were analyzed after the experiment.The body weight,eating and drinking water were examined in each mouse for two weeks.The histopathology of adrenal gland was analyzed and the expression of high mobility group protein(HMGB1)was also determined by immunohistochemistry.The expression of tyrosine hydroxylase(TH)and Anti Iba1 was analyzed with the same way.Results: As compared with the control group,the model group displayed markedly hypoactive behavior.Significant statistical difference was found between two groups in three tests and metabolic index(P<0.05).The proportion of activated microglia in model group is the larger than controls.Histological examination demonstrated severe injuries of the adrenal gland,apparent inflammatory response,thinner cortices with necrosis and unclear boundaries.Immunohistochemical staining showed that the density of HMGB1 stain was increased significantly after treatment with MPTP.Conclusions: Parkinson’s disease may be associated with inflammation,metabolic index and other histopathology changes of adrenal gland,which partially provide a vital new dimension regarding to the conventional treatment for PD.