The Effect Of GCP-2 In A Murine Model Of Pseudomonas Aeruginosa Pneumonia

bjective:By studying the expression of granulocyte chemotactic protein-2 and the Correlation of the granulocyte chemotactic protein-2,interleukin-10 and transforming growth factor-beta 1 in lung tissue in a murine model of pseudomonas aeruginosa pneumonia,the effect of GCP-2 in a murine model of Pseudomonas aeruginosa pneumonia was discussed.A new way to cure the pseudomonas aeruginosa pneumonia was wanted to find.Methods:1.45male kunming mice which were 18-22g were divided into randomly three groups,normal control group,pulmonary infection group(group PA)and the intervention group,each group is 15.Normal control group:firstly modeling before 12 hours through the intraperitoneal injection of physiological saline 2ml;secondly the mice were anesthetized with ketamine(100mg/kg)and then fixed in Aseptic operation table to be effective after anesthesia.we do routine disinfects shop towel,expose upper trachea of mice and inject the saline which is 0.2cm from the tracheal ring.Pulmonary infection group:firstly modeling before 12hours through the intraperitoneal injection of physiological saline 2ml;secondly the mice were anesthetized withketamine(100mg/kg)and then fixed in Aseptic operation table to be effective after anesthesia.we do routine disinfects shop towel,expose upper trachea of mice and inject pseudomonas aeruginosa0.2ml(2 Maxwell unit concentration,6×10~8cfu/ml).The intervention group:firstly modeling before 12 hours through the intraperitoneal injection of physiological saline 2ml which include GCP-2 antibodies 0.15ml(1mg/ml);secondly the mice were anesthetized withketamine(100mg/kg)and then fixed in Aseptic operation table to be effective after anesthesia.we do routine disinfects shop towel,expose upper trachea of mice and inject pseudomonas aeruginosa0.2ml(2 Maxwell unit concentration,6×10~8cfu/ml).2.In the three groups after the success of modeling 5 mice were randomly selected,and taken the specimens and put to death.The bronchoalveolar lavage fluid which come from the right lung of mice were collected at three different times in each group,then centrifuging separation,testing the concentration of GCP-2,IL-10 and TGF-β1 in bronchoalveolar lavage fluid by enzyme linked immunosorbent assay(ELISA).The most left lung was fixed by 10%paraformaldehyde,stained by HE staining,then compare to the range and degree of inflammation.A small part of left lung tissue was made into homogenate for bacterial culture and colony counting.3.SPSS 20.0 software was used for statistical data analysis.Results:1.There is an obvious difference of lung tissue pathological change between the infection group and intervention group mice,the most obvious there exists significant difference with the blank control group,but the intervention group pathological changes is less than the infection group,especially over time increase the more obvious differences between the two groups.The difference of three groups statistically significant(P<0.017).2.The GCP-2 were detected in the alveolar lavage fluid of three groups,GCP-2 expression is most obvious in infection group and intervention group,but the intervention group significantly lower than in the infection group.GCP-2 in infection group expression quantity is higher and higher with time,but after giving GCP-2 antibody the GCP-2 is Less and less over time,the inflammatory response also will reduce at the same time.The difference of three groups statistically significant(P<0.001).3.The IL-10 and TGF-β1 were detected in the alveolar lavage fluid of three groups,IL-10 and TGF-β1expression is most obvious in infection group and intervention group,but the intervention group significantly lower than in the infection group.The function of GCP-2 may relate to the TGF-β1 and IL-10.Conclusions:GCP-2 can promote inflammation,increasing pseudomonas aeruginosa pneumonia in mice.After using GCP-2 antibody,the inflammation of pseudomonas aeruginosa pneumonia in mice can decrease.The function of GCP-2 in mice pseudomonas aeruginosa pneunia may relate to the TGF-β1 and IL-10.