The Establishment And Application Of Swimming Endurance System In Mice

It is extremely important to screen anti-fatigue drugs with positive effects in the pre-clinical studies.In combination with pharmaceutical,computer science and information technology,the swimming endurance system of mice was established.The influence of different weight-load on the endurance in mice was evaluated.Moreover,Hongjingtian,a well known anti-fatigue herb was chose to estimate the fatigue indexes using the weight-loaded swimming test.Besides,influence of different intensity and time running in the cylinder on the swimming endurance in mice was also determined.A swimming endurance system with highly automatic,intelligent and objective in mice has been established.The hardware design of integrated testing jars,with the background light and thermostats as well.Contour collected and double interfaces angles synthesized has been applied in the software of this system,which could capture,collect and then analysis the movement information of the swimming task in mice.On the basis of classic indexes in the weight-loaded swimming test,we introduced a series of new indexes to assess swimming endurance in mice.The correlation coefficient between manual calculation and computer automatic record showed highly stability and reliability of this system.A comparative study on the characteristic of the swimming test on different intensity of weight-loaded was held in mice.Using the established swimming endurance system,the different weight-load(5%-9%)swimming endurance test was performed in ICR mice.The results showed,neither a lower weight-load(5%)nor a higher weight-load(8%)was suitable for the endurance swimming test.Lower weight-load swimming may lead to mice floating on the surface of the water.Higher weight-load swimming may produce severe stress reaction during the test and lead to poor reliable positive results.The result showed the middle weight-load(6%-7%)could act as the desirable choice,because of the suitable exhaustive time,lower stress reaction and the lower coefficient of variation(C.V=0.323-0.369).Application of the swimming endurance system on the effect of Hongjingtian improving weight-load swimming induced fatigue in mice.After the ICR mice intragastric administration Hongjingtian for 14 d,the swimming endurance system was used to determine the swimming endurance in mice.The results not only proved the considerable anti-fatigue effect of Hongjingtian,but also found out two indexes(Fst and Cst)that were more sensitive than the exhaustive time.Application of the swimming endurance system on different intensions of running in the cylinder induced chronic fatigue in mice.Using the cylinder,mice running for 14 days and 28 days respectively with different intensions(4 h/d,8 h/d,16 h/d).The swimming endurance detected using this swimming endurance system test.The result showed that running for 14 days in the cylinder with different intensions had little influence on the endurance of mice.The running for 28 days with different intensions could decrease several indexes including Exhaustive time(ET),Sink frequency(SF),Total sink time(TST),Continuous sink time(CST)in this behavior test,the analysis on the fatigue related biochemical indexes Serum urea nitrogen(SUN)and Serum creatinine(SC)in the serum increased also.The intensions of 4 h/d,8 h/d,16 h/d running for 14 days led to linear rise in serotonin in the brain in mice,and 16 h running group showed significantly increased in the serotonin level when compared with control group.In conclusion,this article introduced a swimming endurance system with highly automatic and intelligent,and could objective assess swimming endurance in mice.The application of this swimming endurance system on weight-loaded swimming test,the anti-fatigue effect of Hongjingtian and different intensions on running in the sylinder induced chronic fatigue were studied.Meanwhile,we found more sensitive indexes when compared with exhaustive time,and we also set up a new chronic fatigue model – running cylingder.Finally,this study provide an automatic,intelligent and objective swimming endurance system,a series of indexes and a new chronic fatigue model which is benefit to screening new anti-fatigue drugs.