Pharmacological Evaluation On The Bitter Flavor Of Cyathula Officinalis Kuan

Radix Cyathula officinalis is from the dried roots of Cyathula officinalis Kuan that belongs to the plant of Cyathula prostrate.The Chinese Pharmacopoeia(published in 2015)records that Radix Cyathula officinalis is sweet taste and a bit bitter,flat property.It can remove blood stasis,make channels dredge and stretch and bend joints freely.In addition,treating obstructed urethra and inducing diuresis is also its efficacy.Rheumatoid arthritis belongs to the part of "Bi syndrome”.Its pathogeny mostly is on account of suffering impact from wind,cold,wet and heat.These factors turn into phlegm and stasis,ultimately will lead to RA.Hence,clinicians mostly treat rheumatoid arthritis on the basis of removing wet and blood clog,improving circulation,relieving pain.From ancient to modern,medical books record that Radix Cyathula officinalis can dispel cold,wet,and stasis and treat pain,injure.Hyperlipidaemia caused by the phlegm and blood stasis.It attacks in blood vessels and veins.In the modern,rising lipid in the blood is called“phlegm stasis of blood”.Doctors treat hyperlipidaemia by the way of getting rid of stasis and improving circulation.All ages,medical books record that Radix Cyathula officinalis can“remove bad blood and stasis”.The research takes Radix Cyathula officinalis as the object according to the new hypothesis-"one medicine has one taste and one nature,one medicine has X tastes and Y natures(Y<X)" in TCM.According to TCM theory,using the methods of philology,pharmacol ogy,analytical chemistry,and so on study the relation between the efficacy of Radix Cyathula officinalis removing wet,stasis,bad blood,disperse stasis and its flavor.Therefore,we accomplish following several aspects of work:1.Reference reviewWe systematically refer to ancient and modern medical books about Radix Cyathula officinalis efficacy of nature,flavor and their application;meanwhile,look up their studing achievements of present-day pharmacology and composition.We complet summarize and the acquaintance of bitter flavor efficacy.2.The preparation of Radix of Cyathula officinalis Kuan total sterone component and study on the quality control Adopting methanol extracts Radix Cyathula offic inali s and separates by macroporous resin SP825L.Determining composition eluted 50%ethanol is total sterone component of Radix Cyathula officinali s through research.We measure the total sterone content of total sterone component by the way of UV-Spectrophotometric and the cyasterone content of total sterone component by HPLC.We conduct methodology examination including linear,accuracy,stablility,reproducibility and the recovery rate.The result of methodology examination indicates that the methods established are feasible.In the experiment,we measure the content of total sterone is 74.8%.;meanwhile,measuring the content of cyasterone is 5 6.0%.3.Pharmacological evaluation on removing wet and blood stasis Bi of Radix of Cyathula officinalis Kuan Using the model of adjuvant arthritis rats and regarding the pathological morphology,paw swelling degree and the content of inflammatory cytokines(IL-1β、IL-6、IL-8、TNF-a)in joint leached liquor and serum as indicators investigates that the total sterone has impacts on joint diseas.The result shows that total sterone can treat RA.4.Pharmacological evaluation on removing bad blood,dispersing stasis of Radix of Cyathula officinalis Kuan Hyperlipidemia model of rats was established by feeding high-fat fodder.Detecting serum fat index of TC、TG、HDL-C、LDL-C、ALT、AST and SOD、MDA,at the same time,determining the liver index and liver pathological section measure the total sterone treatment effect on hyperlipidemia through the above indicators.Results show that the total sterone component can treat hyperlipidemia.Bitter flavor can eliminate dampness and dispel block.Bitter working place particularly stresses on meridian bones and muscle,mostly remove wet and block Bi.Books record that Radix Cyathula officincalis can dispel coldwetstasis.Bitter taste,flat property can make wet,heat,stasis go downside,hence,the relationgship exists between the efficacy of dispeling cold,wet,stasis and bitter taste.Bitter taste sets into the bloodstream and removes phlegm turbidity in the blood.Radix of Cyathula officinalis Kuan is good at invigorating the circulation.The relationgship exists between the efficacy of removing bad blood and dispersing stasis and bitter flavor of Radix of Cyathula officinalis Kuan.Above comprehensive research results include identifying chemical separated component of the TC M Radix of Cyathula officinalis Kuan removing wet,stasis,bad blood,dispersing stasis for the first time,meanwhile,we determine the content of effective material.From the perspective of t he TCM,we explain the relationgship between the efficacy of removing bad blood and disperse stasis and bitter flavor of Radix of Cyathula officinalis Kuan.Preliminarily we determine that total sterone component of Radix of Cyathula officinalis Kuan is probable the material basis of bitter flavor.